Novel Observations From Next-Generation RNA Sequencing of Highly Purified Human Adult and Fetal Islet Cell Subsets
Authors
Blodgett, David M.Nowosielska, Anetta
Afik, Shaked
Pechhold, Susanne
Cura, Anthony J.
Kennedy, Norman J.
Kim, Soyoung
Kucukural, Alper
Davis, Roger J.
Kent, Sally C
Greiner, Dale L.
Garber, Manuel
Harlan, David M.
diLorio, Philip J. Jr.
UMass Chan Affiliations
Program in Bioinformatics and Integrative BiologyDepartment of Biochemistry and Molecular Pharmacology
Program in Molecular Medicine
Diabetes Center of Excellence
Department of Medicine
Document Type
Journal ArticlePublication Date
2015-09-01Keywords
UMCCTS fundingBiochemistry
Bioinformatics
Cell Biology
Computational Biology
Endocrinology, Diabetes, and Metabolism
Integrative Biology
Systems Biology
Metadata
Show full item recordAbstract
Understanding distinct gene expression patterns of normal adult and developing fetal human pancreatic alpha- and beta-cells is crucial for developing stem cell therapies, islet regeneration strategies, and therapies designed to increase beta-cell function in patients with diabetes (type 1 or 2). Toward that end, we have developed methods to highly purify alpha-, beta-, and delta-cells from human fetal and adult pancreata by intracellular staining for the cell-specific hormone content, sorting the subpopulations by flow cytometry, and, using next-generation RNA sequencing, we report the detailed transcriptomes of fetal and adult alpha- and beta-cells. We observed that human islet composition was not influenced by age, sex, or BMI, and transcripts for inflammatory gene products were noted in fetal beta-cells. In addition, within highly purified adult glucagon-expressing alpha-cells, we observed surprisingly high insulin mRNA expression, but not insulin protein expression. This transcriptome analysis from highly purified islet alpha- and beta-cell subsets from fetal and adult pancreata offers clear implications for strategies that seek to increase insulin expression in type 1 and type 2 diabetes. long as the work is properly cited, the use is educational and not for profit, and the work is not altered.Source
Diabetes. 2015 Sep;64(9):3172-81. doi: 10.2337/db15-0039. Epub 2015 Apr 30. Link to article on publisher's site
DOI
10.2337/db15-0039Permanent Link to this Item
http://hdl.handle.net/20.500.14038/25935PubMed ID
25931473Related Resources
ae974a485f413a2113503eed53cd6c53
10.2337/db15-0039