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dc.contributor.authorBlodgett, David M.
dc.contributor.authorNowosielska, Anetta
dc.contributor.authorAfik, Shaked
dc.contributor.authorPechhold, Susanne
dc.contributor.authorCura, Anthony J.
dc.contributor.authorKennedy, Norman J.
dc.contributor.authorKim, Soyoung
dc.contributor.authorKucukural, Alper
dc.contributor.authorDavis, Roger J.
dc.contributor.authorKent, Sally C
dc.contributor.authorGreiner, Dale L.
dc.contributor.authorGarber, Manuel
dc.contributor.authorHarlan, David M.
dc.contributor.authordiLorio, Philip J. Jr.
dc.date2022-08-11T08:07:59.000
dc.date.accessioned2022-08-23T15:38:26Z
dc.date.available2022-08-23T15:38:26Z
dc.date.issued2015-09-01
dc.date.submitted2016-01-07
dc.identifier.citation<p>Diabetes. 2015 Sep;64(9):3172-81. doi: 10.2337/db15-0039. Epub 2015 Apr 30. <a href="http://dx.doi.org/10.2337/db15-0039">Link to article on publisher's site</a></p>
dc.identifier.issn0012-1797 (Linking)
dc.identifier.doi10.2337/db15-0039
dc.identifier.pmid25931473
dc.identifier.urihttp://hdl.handle.net/20.500.14038/25935
dc.description.abstractUnderstanding distinct gene expression patterns of normal adult and developing fetal human pancreatic alpha- and beta-cells is crucial for developing stem cell therapies, islet regeneration strategies, and therapies designed to increase beta-cell function in patients with diabetes (type 1 or 2). Toward that end, we have developed methods to highly purify alpha-, beta-, and delta-cells from human fetal and adult pancreata by intracellular staining for the cell-specific hormone content, sorting the subpopulations by flow cytometry, and, using next-generation RNA sequencing, we report the detailed transcriptomes of fetal and adult alpha- and beta-cells. We observed that human islet composition was not influenced by age, sex, or BMI, and transcripts for inflammatory gene products were noted in fetal beta-cells. In addition, within highly purified adult glucagon-expressing alpha-cells, we observed surprisingly high insulin mRNA expression, but not insulin protein expression. This transcriptome analysis from highly purified islet alpha- and beta-cell subsets from fetal and adult pancreata offers clear implications for strategies that seek to increase insulin expression in type 1 and type 2 diabetes. long as the work is properly cited, the use is educational and not for profit, and the work is not altered.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=25931473&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttp://dx.doi.org/10.2337/db15-0039
dc.subjectUMCCTS funding
dc.subjectBiochemistry
dc.subjectBioinformatics
dc.subjectCell Biology
dc.subjectComputational Biology
dc.subjectEndocrinology, Diabetes, and Metabolism
dc.subjectIntegrative Biology
dc.subjectSystems Biology
dc.titleNovel Observations From Next-Generation RNA Sequencing of Highly Purified Human Adult and Fetal Islet Cell Subsets
dc.typeJournal Article
dc.source.journaltitleDiabetes
dc.source.volume64
dc.source.issue9
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/bioinformatics_pubs/76
dc.identifier.contextkey7992044
html.description.abstract<p>Understanding distinct gene expression patterns of normal adult and developing fetal human pancreatic alpha- and beta-cells is crucial for developing stem cell therapies, islet regeneration strategies, and therapies designed to increase beta-cell function in patients with diabetes (type 1 or 2). Toward that end, we have developed methods to highly purify alpha-, beta-, and delta-cells from human fetal and adult pancreata by intracellular staining for the cell-specific hormone content, sorting the subpopulations by flow cytometry, and, using next-generation RNA sequencing, we report the detailed transcriptomes of fetal and adult alpha- and beta-cells. We observed that human islet composition was not influenced by age, sex, or BMI, and transcripts for inflammatory gene products were noted in fetal beta-cells. In addition, within highly purified adult glucagon-expressing alpha-cells, we observed surprisingly high insulin mRNA expression, but not insulin protein expression. This transcriptome analysis from highly purified islet alpha- and beta-cell subsets from fetal and adult pancreata offers clear implications for strategies that seek to increase insulin expression in type 1 and type 2 diabetes. long as the work is properly cited, the use is educational and not for profit, and the work is not altered.</p>
dc.identifier.submissionpathbioinformatics_pubs/76
dc.contributor.departmentProgram in Bioinformatics and Integrative Biology
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentDiabetes Center of Excellence
dc.contributor.departmentDepartment of Medicine
dc.source.pages3172-81


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