Show simple item record

Functional annotation of native enhancers with a Cas9-histone demethylase fusion

dc.contributor.authorKearns, Nicola A.
dc.contributor.authorPham, Hannah
dc.contributor.authorTabak, Barbara
dc.contributor.authorGenga, Ryan M. J.
dc.contributor.authorSilverstein, Noah J.
dc.contributor.authorGarber, Manuel
dc.contributor.authorMaehr, Rene
dc.date2022-08-11T08:07:59.000
dc.date.accessioned2022-08-23T15:38:27Z
dc.date.available2022-08-23T15:38:27Z
dc.date.issued2015-05-01
dc.date.submitted2016-01-07
dc.identifier.citation<p>Nat Methods. 2015 May;12(5):401-3. doi: 10.1038/nmeth.3325. Epub 2015 Mar 16. <a href="http://dx.doi.org/10.1038/nmeth.3325">Link to article on publisher's site</a></p>
dc.identifier.issn1548-7091 (Linking)
dc.identifier.doi10.1038/nmeth.3325
dc.identifier.pmid25775043
dc.identifier.urihttp://hdl.handle.net/20.500.14038/25938
dc.description.abstractUnderstanding of mammalian enhancers is limited by the lack of a technology to rapidly and thoroughly test the cell type-specific function. Here, we use a nuclease-deficient Cas9 (dCas9)-histone demethylase fusion to functionally characterize previously described and new enhancer elements for their roles in the embryonic stem cell state. Further, we distinguish the mechanism of action of dCas9-LSD1 at enhancers from previous dCas9-effectors.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=25775043&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414811/
dc.subjectAnimals
dc.subjectBacterial Proteins
dc.subjectCaspase 9
dc.subjectCells, Cultured
dc.subjectEmbryonic Stem Cells
dc.subjectEnhancer Elements, Genetic
dc.subjectGene Expression Regulation, Enzymologic
dc.subjectGenes, Reporter
dc.subjectHistone Demethylases
dc.subjectHistones
dc.subjectMice
dc.subjectNeisseria meningitidis
dc.subjectRecombinant Proteins
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.subjectBioinformatics
dc.subjectComputational Biology
dc.subjectIntegrative Biology
dc.subjectSystems Biology
dc.titleFunctional annotation of native enhancers with a Cas9-histone demethylase fusion
dc.typeJournal Article
dc.source.journaltitleNature methods
dc.source.volume12
dc.source.issue5
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/bioinformatics_pubs/79
dc.identifier.contextkey7992047
html.description.abstract<p>Understanding of mammalian enhancers is limited by the lack of a technology to rapidly and thoroughly test the cell type-specific function. Here, we use a nuclease-deficient Cas9 (dCas9)-histone demethylase fusion to functionally characterize previously described and new enhancer elements for their roles in the embryonic stem cell state. Further, we distinguish the mechanism of action of dCas9-LSD1 at enhancers from previous dCas9-effectors.</p>
dc.identifier.submissionpathbioinformatics_pubs/79
dc.contributor.departmentProgram in Bioinformatics and Integrative Biology
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentDiabetes Center of Excellence
dc.source.pages401-3


This item appears in the following Collection(s)

Show simple item record