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dc.contributor.authorFukunaga, Ryuya
dc.contributor.authorHan, Bo W.
dc.contributor.authorHung, Jui-Hung
dc.contributor.authorXu, Jia
dc.contributor.authorWeng, Zhiping
dc.contributor.authorZamore, Phillip D.
dc.date2022-08-11T08:07:59.000
dc.date.accessioned2022-08-23T15:38:27Z
dc.date.available2022-08-23T15:38:27Z
dc.date.issued2012-10-26
dc.date.submitted2013-02-22
dc.identifier.citationCell. 2012 Oct 26;151(3):533-46. doi: 10.1016/j.cell.2012.09.027. <a href="http://dx.doi.org/10.1016/j.cell.2012.09.027">Link to article on publisher's site</a>
dc.identifier.issn0092-8674 (Linking)
dc.identifier.doi10.1016/j.cell.2012.09.027
dc.identifier.pmid23063653
dc.identifier.urihttp://hdl.handle.net/20.500.14038/25939
dc.description.abstractDrosophila Dicer-1 produces microRNAs (miRNAs) from pre-miRNA, whereas Dicer-2 generates small interfering RNAs (siRNAs) from long dsRNA. Alternative splicing of the loquacious (loqs) mRNA generates three distinct Dicer partner proteins. To understand the function of each, we constructed flies expressing Loqs-PA, Loqs-PB, or Loqs-PD. Loqs-PD promotes both endo- and exo-siRNA production by Dicer-2. Loqs-PA or Loqs-PB is required for viability, but the proteins are not fully redundant: a specific subset of miRNAs requires Loqs-PB. Surprisingly, Loqs-PB tunes where Dicer-1 cleaves pre-miR-307a, generating a longer miRNA isoform with a distinct seed sequence and target specificity. The longer form of miR-307a represses glycerol kinase and taranis mRNA expression. The mammalian Dicer-partner TRBP, a Loqs-PB homolog, similarly tunes where Dicer cleaves pre-miR-132. Thus, Dicer-binding partner proteins change the choice of cleavage site by Dicer, producing miRNAs with target specificities different from those made by Dicer alone or Dicer bound to alternative protein partners.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=23063653&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3609031/
dc.subjectAnimals
dc.subjectBase Sequence
dc.subjectDEAD-box RNA Helicases
dc.subjectDrosophila Proteins
dc.subjectDrosophila melanogaster
dc.subjectFemale
dc.subjectHumans
dc.subjectMale
dc.subjectMice
dc.subjectMicroRNAs
dc.subjectMolecular Sequence Data
dc.subjectRNA Helicases
dc.subjectRNA-Binding Proteins
dc.subjectRibonuclease III
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.subjectBioinformatics
dc.subjectMolecular Biology
dc.titleDicer partner proteins tune the length of mature miRNAs in flies and mammals
dc.typeJournal Article
dc.source.journaltitleCell
dc.source.volume151
dc.source.issue3
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/bioinformatics_pubs/8
dc.identifier.contextkey3761389
html.description.abstract<p>Drosophila Dicer-1 produces microRNAs (miRNAs) from pre-miRNA, whereas Dicer-2 generates small interfering RNAs (siRNAs) from long dsRNA. Alternative splicing of the loquacious (loqs) mRNA generates three distinct Dicer partner proteins. To understand the function of each, we constructed flies expressing Loqs-PA, Loqs-PB, or Loqs-PD. Loqs-PD promotes both endo- and exo-siRNA production by Dicer-2. Loqs-PA or Loqs-PB is required for viability, but the proteins are not fully redundant: a specific subset of miRNAs requires Loqs-PB. Surprisingly, Loqs-PB tunes where Dicer-1 cleaves pre-miR-307a, generating a longer miRNA isoform with a distinct seed sequence and target specificity. The longer form of miR-307a represses glycerol kinase and taranis mRNA expression. The mammalian Dicer-partner TRBP, a Loqs-PB homolog, similarly tunes where Dicer cleaves pre-miR-132. Thus, Dicer-binding partner proteins change the choice of cleavage site by Dicer, producing miRNAs with target specificities different from those made by Dicer alone or Dicer bound to alternative protein partners.</p>
dc.identifier.submissionpathbioinformatics_pubs/8
dc.contributor.departmentProgram in Bioinformatics and Integrative Biology
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.source.pages533-46


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