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dc.contributor.authorTang, Wen
dc.contributor.authorTu, Shikui
dc.contributor.authorLee, Heng-Chi
dc.contributor.authorWeng, Zhiping
dc.contributor.authorMello, Craig C.
dc.date2022-08-11T08:07:59.000
dc.date.accessioned2022-08-23T15:38:28Z
dc.date.available2022-08-23T15:38:28Z
dc.date.issued2016-02-25
dc.date.submitted2016-04-14
dc.identifier.citationCell. 2016 Feb 25;164(5):974-84. doi: 10.1016/j.cell.2016.02.008. <a href="http://dx.doi.org/10.1016/j.cell.2016.02.008">Link to article on publisher's site</a>
dc.identifier.issn0092-8674 (Linking)
dc.identifier.doi10.1016/j.cell.2016.02.008
dc.identifier.pmid26919432
dc.identifier.urihttp://hdl.handle.net/20.500.14038/25942
dc.description.abstractPiwi-interacting RNAs (piRNAs) engage Piwi proteins to suppress transposons and are essential for fertility in diverse organisms. An interesting feature of piRNAs is that, while piRNA lengths are stereotypical within a species, they can differ widely between species. For example, piRNAs are mainly 29 and 30 nucleotides in humans, 24 to 30 nucleotides in D. melanogaster, and uniformly 21 nucleotides in C. elegans. However, how piRNA length is determined and whether length impacts function remains unknown. Here, we show that C. elegans deficient for PARN-1, a conserved RNase, accumulate untrimmed piRNAs with 3' extensions. Surprisingly, these longer piRNAs are stable and associate with the Piwi protein PRG-1 but fail to robustly recruit downstream silencing factors. Our findings identify PARN-1 as a key regulator of piRNA length in C. elegans and suggest that length is regulated to promote efficient transcriptome surveillance.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=26919432&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1016/j.cell.2016.02.008
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.subjectBioinformatics
dc.subjectCell Biology
dc.subjectComputational Biology
dc.subjectIntegrative Biology
dc.subjectSystems Biology
dc.titleThe RNase PARN-1 Trims piRNA 3' Ends to Promote Transcriptome Surveillance in C. elegans
dc.typeArticle
dc.source.journaltitleCell
dc.source.volume164
dc.source.issue5
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/bioinformatics_pubs/82
dc.identifier.contextkey8478477
html.description.abstract<p>Piwi-interacting RNAs (piRNAs) engage Piwi proteins to suppress transposons and are essential for fertility in diverse organisms. An interesting feature of piRNAs is that, while piRNA lengths are stereotypical within a species, they can differ widely between species. For example, piRNAs are mainly 29 and 30 nucleotides in humans, 24 to 30 nucleotides in D. melanogaster, and uniformly 21 nucleotides in C. elegans. However, how piRNA length is determined and whether length impacts function remains unknown. Here, we show that C. elegans deficient for PARN-1, a conserved RNase, accumulate untrimmed piRNAs with 3' extensions. Surprisingly, these longer piRNAs are stable and associate with the Piwi protein PRG-1 but fail to robustly recruit downstream silencing factors. Our findings identify PARN-1 as a key regulator of piRNA length in C. elegans and suggest that length is regulated to promote efficient transcriptome surveillance.</p>
dc.identifier.submissionpathbioinformatics_pubs/82
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentProgram in Bioinformatics and Integrative Biology
dc.contributor.departmentRNA Therapeutics Institute
dc.source.pages974-84


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