UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyProgram in Bioinformatics and Integrative Biology
Document Type
Journal ArticlePublication Date
2016-11-01
Metadata
Show full item recordAbstract
This protocol illustrates the steps of searching a sequence database with BLAST, which in essence performs pairwise alignment between the query sequence and each target sequence in the database. Steps for performing multiple sequence alignments with ClustalW are then described. Argonaute 2 (Ago2) is an essential component for small interfering RNA (siRNA)-directed RNA interference (RNAi) in fruit flies. This protocol uses Ago2 to demonstrate how to search for all Ago2 paralogs in the fly proteome using BLAST and to obtain a multiple sequence alignment of these sequences with ClustalW.Source
Cold Spring Harb Protoc. 2016 Nov 1;2016(11):pdb.prot093088. doi: 10.1101/pdb.prot093088. Link to article on publisher's siteDOI
10.1101/pdb.prot093088Permanent Link to this Item
http://hdl.handle.net/20.500.14038/25954PubMed ID
27574197Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1101/pdb.prot093088