Show simple item record

dc.contributor.authorTsuji, Junko
dc.contributor.authorThomson, Travis
dc.contributor.authorBrown, Christine
dc.contributor.authorGhosh, Subhanita
dc.contributor.authorTheurkauf, William E.
dc.contributor.authorWeng, Zhiping
dc.contributor.authorSchwartz, Lawrence M.
dc.date2022-08-11T08:08:00.000
dc.date.accessioned2022-08-23T15:38:34Z
dc.date.available2022-08-23T15:38:34Z
dc.date.issued2021-12-22
dc.date.submitted2022-07-05
dc.identifier.citation<p>Tsuji J, Thomson T, Brown C, Ghosh S, Theurkauf WE, Weng Z, Schwartz LM. Somatic piRNAs and Transposons are Differentially Expressed Coincident with Skeletal Muscle Atrophy and Programmed Cell Death. Front Genet. 2021 Dec 22;12:775369. doi: 10.3389/fgene.2021.775369. PMID: 35003216; PMCID: PMC8730325. <a href="https://doi.org/10.3389/fgene.2021.775369">Link to article on publisher's site</a></p>
dc.identifier.issn1664-8021 (Linking)
dc.identifier.doi10.3389/fgene.2021.775369
dc.identifier.pmid35003216
dc.identifier.urihttp://hdl.handle.net/20.500.14038/25962
dc.description.abstractPIWI-interacting RNAs (piRNAs) are small single-stranded RNAs that can repress transposon expression via epigenetic silencing and transcript degradation. They have been identified predominantly in the ovary and testis, where they serve essential roles in transposon silencing in order to protect the integrity of the genome in the germline. The potential expression of piRNAs in somatic cells has been controversial. In the present study we demonstrate the expression of piRNAs derived from both genic and transposon RNAs in the intersegmental muscles (ISMs) from the tobacco hawkmoth Manduca sexta. These piRNAs are abundantly expressed, approximately 27 nt long, map antisense to transposons, are oxidation resistant, exhibit a 5' uridine bias, and amplify via the canonical ping-pong pathway. An RNA-seq analysis demonstrated that 19 piRNA pathway genes are expressed in the ISMs and are developmentally regulated. The abundance of piRNAs does not change when the muscles initiate developmentally-regulated atrophy, but are repressed coincident with the commitment of the muscles undergo programmed cell death at the end of metamorphosis. This change in piRNA expression is correlated with the repression of several retrotransposons and the induction of specific DNA transposons. The developmentally-regulated changes in the expression of piRNAs, piRNA pathway genes, and transposons are all regulated by 20-hydroxyecdysone, the steroid hormone that controls the timing of ISM death. Taken together, these data provide compelling evidence for the existence of piRNA in somatic tissues and suggest that they may play roles in developmental processes such as programmed cell death.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=35003216&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright © 2021 Tsuji, Thomson, Brown, Ghosh, Theurkauf, Weng and Schwartz. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectManduca sexta
dc.subjectRNA interference
dc.subjectdevelopment
dc.subjectping-pong amplification
dc.subjectsmall RNAs
dc.subjecttransposon
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.subjectBioinformatics
dc.subjectCell Biology
dc.subjectComputational Biology
dc.subjectDevelopmental Biology
dc.subjectGenetics and Genomics
dc.subjectSystems Biology
dc.titleSomatic piRNAs and Transposons are Differentially Expressed Coincident with Skeletal Muscle Atrophy and Programmed Cell Death
dc.typeJournal Article
dc.source.journaltitleFrontiers in genetics
dc.source.volume12
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1184&amp;context=bioinformatics_pubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/bioinformatics_pubs/173
dc.identifier.contextkey30064403
refterms.dateFOA2022-08-23T15:38:34Z
html.description.abstract<p>PIWI-interacting RNAs (piRNAs) are small single-stranded RNAs that can repress transposon expression via epigenetic silencing and transcript degradation. They have been identified predominantly in the ovary and testis, where they serve essential roles in transposon silencing in order to protect the integrity of the genome in the germline. The potential expression of piRNAs in somatic cells has been controversial. In the present study we demonstrate the expression of piRNAs derived from both genic and transposon RNAs in the intersegmental muscles (ISMs) from the tobacco hawkmoth Manduca sexta. These piRNAs are abundantly expressed, approximately 27 nt long, map antisense to transposons, are oxidation resistant, exhibit a 5' uridine bias, and amplify via the canonical ping-pong pathway. An RNA-seq analysis demonstrated that 19 piRNA pathway genes are expressed in the ISMs and are developmentally regulated. The abundance of piRNAs does not change when the muscles initiate developmentally-regulated atrophy, but are repressed coincident with the commitment of the muscles undergo programmed cell death at the end of metamorphosis. This change in piRNA expression is correlated with the repression of several retrotransposons and the induction of specific DNA transposons. The developmentally-regulated changes in the expression of piRNAs, piRNA pathway genes, and transposons are all regulated by 20-hydroxyecdysone, the steroid hormone that controls the timing of ISM death. Taken together, these data provide compelling evidence for the existence of piRNA in somatic tissues and suggest that they may play roles in developmental processes such as programmed cell death.</p>
dc.identifier.submissionpathbioinformatics_pubs/173
dc.contributor.departmentNeurobiology
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentProgram in Bioinformatics and Integrative Biology
dc.source.pages775369


Files in this item

Thumbnail
Name:
fgene_12_775369.pdf
Size:
1.646Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record

Copyright © 2021 Tsuji, Thomson, Brown, Ghosh, Theurkauf, Weng and Schwartz. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Except where otherwise noted, this item's license is described as Copyright © 2021 Tsuji, Thomson, Brown, Ghosh, Theurkauf, Weng and Schwartz. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.