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Pleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K12

dc.contributor.authorMarinus, Martin G.
dc.contributor.authorMorris, N. Ronald
dc.date2022-08-11T08:08:00.000
dc.date.accessioned2022-08-23T15:38:39Z
dc.date.available2022-08-23T15:38:39Z
dc.date.issued1975-04-01
dc.date.submitted2009-01-08
dc.identifier.citationMutat Res. 1975 Apr;28(1):15-26.
dc.identifier.issn0027-5107 (Print)
dc.identifier.pmid167279
dc.identifier.urihttp://hdl.handle.net/20.500.14038/25981
dc.description.abstractThe dam-3 mutation results in a five-fold reduction in the number of 6-methyl-adenine (6-meA) residues in the DNA of E. coli K12 or phage lambda. The DNA of phage fd appears to be devoid of 6-meA when propagated on dam-3 bacteria. The phenotypic differences between dam-3 and dam+ bacteria include: (i) increased free phage in lysogenic dam-3 cultures, (2) increased sensitivity to methyl methanesulfonate (MMS), (3) inviability of dam-3 lex-I strains, (4) lower molecular weight of DNA in dam-3 bacteria in the absence of DNA ligase and (5) increased rate of DNA degradation in dam-3 recA strains.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=167279&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1016/0027-5107(75)90309-7
dc.subjectAdenine
dc.subjectCentrifugation, Zonal
dc.subjectColiphages
dc.subjectConjugation, Genetic
dc.subjectDNA Repair
dc.subject*DNA, Bacterial
dc.subjectDNA, Viral
dc.subjectEscherichia coli
dc.subjectLysogeny
dc.subjectMesylates
dc.subjectMethylation
dc.subjectMolecular Weight
dc.subject*Mutation
dc.subjectPhenotype
dc.subjectRadiation Genetics
dc.subjectTransduction, Genetic
dc.subjectUltraviolet Rays
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.titlePleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K12
dc.typeJournal Article
dc.source.journaltitleMutation research
dc.source.volume28
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/bmp_pp/11
dc.identifier.contextkey690837
html.description.abstract<p>The dam-3 mutation results in a five-fold reduction in the number of 6-methyl-adenine (6-meA) residues in the DNA of E. coli K12 or phage lambda. The DNA of phage fd appears to be devoid of 6-meA when propagated on dam-3 bacteria. The phenotypic differences between dam-3 and dam+ bacteria include: (i) increased free phage in lysogenic dam-3 cultures, (2) increased sensitivity to methyl methanesulfonate (MMS), (3) inviability of dam-3 lex-I strains, (4) lower molecular weight of DNA in dam-3 bacteria in the absence of DNA ligase and (5) increased rate of DNA degradation in dam-3 recA strains.</p>
dc.identifier.submissionpathbmp_pp/11
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.source.pages15-26


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