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    Inhibition of aggregation by media selection, sample loading and elution in size exclusion chromatographic refolding of denatured bovine carbonic anhydrase B

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    Authors
    Gu, Zhenyu
    Zhu, Xiaonan
    Ni, Shaowei
    Zhou, Haimeng
    Su, Zhiguo
    UMass Chan Affiliations
    Program in Molecular Medicine
    Department of Biochemistry and Molecular Pharmacology
    Document Type
    Journal Article
    Publication Date
    2003-07-02
    Keywords
    Animals
    Carbonic Anhydrase I
    Cattle
    Chromatography, Gel
    Enzyme Activation
    Gels
    Macromolecular Substances
    Microfluidics
    *Protein Binding
    Protein Denaturation
    *Protein Folding
    Biochemistry, Biophysics, and Structural Biology
    Pharmacology, Toxicology and Environmental Health
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    Link to Full Text
    http://dx.doi.org/10.1016/S0165-022X(03)00057-5
    Abstract
    The effects of gel media, sample application and elution flowrate on the activity recovery and aggregation in refolding of bovine carbonic anhydrase B (CAB) by size exclusion chromatography (SEC) were investigated. Variation in aggregation was demonstrated visibly by the comparison of refolding profiles under different operating conditions. Some principles with regard to practical application were proposed. Meanwhile, the analysis of relationship between peak resolution and activity recovery provided evidences for the mechanism of size exclusion chromatography protein refolding.
    Source
    J Biochem Biophys Methods. 2003 Jun 30;56(1-3):165-75.
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/25987
    PubMed ID
    12834975
    Related Resources
    Link to Article in PubMed
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    UMass Chan Faculty and Researcher Publications

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