Characterization of DNA adenine methylation mutants of Escherichia coli K12
| dc.contributor.author | Bale, Allen | |
| dc.contributor.author | d'Alarcao, Marc | |
| dc.contributor.author | Marinus, Martin G. | |
| dc.date | 2022-08-11T08:08:00.000 | |
| dc.date.accessioned | 2022-08-23T15:38:47Z | |
| dc.date.available | 2022-08-23T15:38:47Z | |
| dc.date.issued | 1979-02-01 | |
| dc.date.submitted | 2009-01-08 | |
| dc.identifier.citation | Mutat Res. 1979 Feb;59(2):157-65. | |
| dc.identifier.issn | 0027-5107 (Print) | |
| dc.identifier.pmid | 375073 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.14038/26010 | |
| dc.description.abstract | The phenotypic traits of 7 independently isolated dam mutants of Escherichia coli have been examined. The mutant strains differ from the wildtype in the following respects: (1) decreased DNA adenine methylase activity in vivo and in vitro; (2) a 14--85-fold increase in spontaneous mutability; (3) decreased survival after ultraviolet irradiation; (4) a 10--21-fold increase in spontaneous induction of lambda phage from lysogens; (5) a 3--17-fold increase in the level of recombination; and (6) inviability of double mutants containing dam- and recB- or recC-. Unmethylated fd phage chromosomes are able to replicate normally in dam- mutants. A mutant strain in which the dcm gene is deleted is viable, showing that the dcm gene product is dispensible for growth. | |
| dc.language.iso | en_US | |
| dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=375073&dopt=Abstract">Link to Article in PubMed</a> | |
| dc.relation.url | http://dx.doi.org/10.1016/0027-5107(79)90153-2 | |
| dc.subject | Adenine | |
| dc.subject | Conjugation, Genetic | |
| dc.subject | DNA (Cytosine-5-)-Methyltransferase | |
| dc.subject | DNA, Bacterial | |
| dc.subject | Escherichia coli | |
| dc.subject | Genotype | |
| dc.subject | Methyltransferases | |
| dc.subject | *Mutation | |
| dc.subject | Phenotype | |
| dc.subject | Recombination, Genetic | |
| dc.subject | Transduction, Genetic | |
| dc.subject | Biochemistry, Biophysics, and Structural Biology | |
| dc.title | Characterization of DNA adenine methylation mutants of Escherichia coli K12 | |
| dc.type | Journal Article | |
| dc.source.journaltitle | Mutation research | |
| dc.source.volume | 59 | |
| dc.source.issue | 2 | |
| dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/bmp_pp/14 | |
| dc.identifier.contextkey | 690840 | |
| html.description.abstract | <p>The phenotypic traits of 7 independently isolated dam mutants of Escherichia coli have been examined. The mutant strains differ from the wildtype in the following respects: (1) decreased DNA adenine methylase activity in vivo and in vitro; (2) a 14--85-fold increase in spontaneous mutability; (3) decreased survival after ultraviolet irradiation; (4) a 10--21-fold increase in spontaneous induction of lambda phage from lysogens; (5) a 3--17-fold increase in the level of recombination; and (6) inviability of double mutants containing dam- and recB- or recC-. Unmethylated fd phage chromosomes are able to replicate normally in dam- mutants. A mutant strain in which the dcm gene is deleted is viable, showing that the dcm gene product is dispensible for growth.</p> | |
| dc.identifier.submissionpath | bmp_pp/14 | |
| dc.contributor.department | Department of Biochemistry and Molecular Pharmacology | |
| dc.source.pages | 157-65 |