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    Heterotypic piRNA Ping-Pong requires qin, a protein with both E3 ligase and Tudor domains

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    Authors
    Zhang, Zhao
    Xu, Jia
    Koppetsch, Birgit S.
    Wang, Jie
    Tipping, Cindy
    Ma, Shengmei
    Weng, Zhiping
    Theurkauf, William E.
    Zamore, Phillip D.
    UMass Chan Affiliations
    Program in Molecular Medicine
    Program in Bioinformatics and Integrative Biology
    Department of Biochemistry and Molecular Pharmacology
    Document Type
    Journal Article
    Publication Date
    2011-11-18
    Keywords
    Animals
    Argonaute Proteins
    Cell Nucleus
    Computational Biology
    DNA Damage
    DNA Transposable Elements
    Drosophila Proteins
    Drosophila melanogaster
    Female
    Fertility
    *Gene Silencing
    *Genome, Insect
    Membrane Transport Proteins
    Mutation
    Oocytes
    Ovary
    Peptide Initiation Factors
    Protein Structure, Tertiary
    RNA Cleavage
    RNA, Small Interfering
    RNA-Induced Silencing Complex
    Signal Transduction
    Ubiquitin-Protein Ligases
    UMCCTS funding
    Biochemistry, Biophysics, and Structural Biology
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    Link to Full Text
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236501
    Abstract
    piRNAs guide PIWI proteins to silence transposons in animal germ cells. Reciprocal cycles of piRNA-directed RNA cleavage--catalyzed by the PIWI proteins Aubergine (Aub) and Argonaute3 (Ago3) in Drosophila melanogaster--expand the population of antisense piRNAs in response to transposon expression, a process called the Ping-Pong cycle. Heterotypic Ping-Pong between Aub and Ago3 ensures that antisense piRNAs predominate. We show that qin, a piRNA pathway gene whose protein product contains both E3 ligase and Tudor domains, colocalizes with Aub and Ago3 in nuage, a perinuclear structure implicated in transposon silencing. In qin mutants, less Ago3 binds Aub, futile Aub:Aub homotypic Ping-Pong prevails, antisense piRNAs decrease, many families of mobile genetic elements are reactivated, and DNA damage accumulates in nurse cells and oocytes. We propose that Qin enforces heterotypic Ping-Pong between Aub and Ago3, ensuring that transposons are silenced and maintaining the integrity of the germline genome.
    Source

    Mol Cell. 2011 Nov 18;44(4):572-84. doi: 10.1016/j.molcel.2011.10.011. Link to article on publisher's site

    DOI
    10.1016/j.molcel.2011.10.011
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/26025
    PubMed ID
    22099305
    Notes

    Author Zhao Zhang is a student in the Interdisciplinary Graduate Program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.

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    10.1016/j.molcel.2011.10.011
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