Heterotypic piRNA Ping-Pong requires qin, a protein with both E3 ligase and Tudor domains
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Authors
Zhang, ZhaoXu, Jia
Koppetsch, Birgit S.
Wang, Jie
Tipping, Cindy
Ma, Shengmei
Weng, Zhiping
Theurkauf, William E.
Zamore, Phillip D.
UMass Chan Affiliations
Program in Molecular MedicineProgram in Bioinformatics and Integrative Biology
Department of Biochemistry and Molecular Pharmacology
Document Type
Journal ArticlePublication Date
2011-11-18Keywords
AnimalsArgonaute Proteins
Cell Nucleus
Computational Biology
DNA Damage
DNA Transposable Elements
Drosophila Proteins
Drosophila melanogaster
Female
Fertility
*Gene Silencing
*Genome, Insect
Membrane Transport Proteins
Mutation
Oocytes
Ovary
Peptide Initiation Factors
Protein Structure, Tertiary
RNA Cleavage
RNA, Small Interfering
RNA-Induced Silencing Complex
Signal Transduction
Ubiquitin-Protein Ligases
UMCCTS funding
Biochemistry, Biophysics, and Structural Biology
Metadata
Show full item recordAbstract
piRNAs guide PIWI proteins to silence transposons in animal germ cells. Reciprocal cycles of piRNA-directed RNA cleavage--catalyzed by the PIWI proteins Aubergine (Aub) and Argonaute3 (Ago3) in Drosophila melanogaster--expand the population of antisense piRNAs in response to transposon expression, a process called the Ping-Pong cycle. Heterotypic Ping-Pong between Aub and Ago3 ensures that antisense piRNAs predominate. We show that qin, a piRNA pathway gene whose protein product contains both E3 ligase and Tudor domains, colocalizes with Aub and Ago3 in nuage, a perinuclear structure implicated in transposon silencing. In qin mutants, less Ago3 binds Aub, futile Aub:Aub homotypic Ping-Pong prevails, antisense piRNAs decrease, many families of mobile genetic elements are reactivated, and DNA damage accumulates in nurse cells and oocytes. We propose that Qin enforces heterotypic Ping-Pong between Aub and Ago3, ensuring that transposons are silenced and maintaining the integrity of the germline genome.Source
Mol Cell. 2011 Nov 18;44(4):572-84. doi: 10.1016/j.molcel.2011.10.011. Link to article on publisher's site
DOI
10.1016/j.molcel.2011.10.011Permanent Link to this Item
http://hdl.handle.net/20.500.14038/26025PubMed ID
22099305Notes
Author Zhao Zhang is a student in the Interdisciplinary Graduate Program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.
Related Resources
ae974a485f413a2113503eed53cd6c53
10.1016/j.molcel.2011.10.011
Scopus Count
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