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dc.contributor.authorZhang, Zhao
dc.contributor.authorXu, Jia
dc.contributor.authorKoppetsch, Birgit S.
dc.contributor.authorWang, Jie
dc.contributor.authorTipping, Cindy
dc.contributor.authorMa, Shengmei
dc.contributor.authorWeng, Zhiping
dc.contributor.authorTheurkauf, William E.
dc.contributor.authorZamore, Phillip D.
dc.date2022-08-11T08:08:00.000
dc.date.accessioned2022-08-23T15:38:51Z
dc.date.available2022-08-23T15:38:51Z
dc.date.issued2011-11-18
dc.date.submitted2013-01-04
dc.identifier.citation<p>Mol Cell. 2011 Nov 18;44(4):572-84. doi: 10.1016/j.molcel.2011.10.011. <a href="http://dx.doi.org/10.1016/j.molcel.2011.10.011">Link to article on publisher's site</a></p>
dc.identifier.issn1097-2765 (Linking)
dc.identifier.doi10.1016/j.molcel.2011.10.011
dc.identifier.pmid22099305
dc.identifier.urihttp://hdl.handle.net/20.500.14038/26025
dc.description<p>Author Zhao Zhang is a student in the Interdisciplinary Graduate Program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.</p>
dc.description.abstractpiRNAs guide PIWI proteins to silence transposons in animal germ cells. Reciprocal cycles of piRNA-directed RNA cleavage--catalyzed by the PIWI proteins Aubergine (Aub) and Argonaute3 (Ago3) in Drosophila melanogaster--expand the population of antisense piRNAs in response to transposon expression, a process called the Ping-Pong cycle. Heterotypic Ping-Pong between Aub and Ago3 ensures that antisense piRNAs predominate. We show that qin, a piRNA pathway gene whose protein product contains both E3 ligase and Tudor domains, colocalizes with Aub and Ago3 in nuage, a perinuclear structure implicated in transposon silencing. In qin mutants, less Ago3 binds Aub, futile Aub:Aub homotypic Ping-Pong prevails, antisense piRNAs decrease, many families of mobile genetic elements are reactivated, and DNA damage accumulates in nurse cells and oocytes. We propose that Qin enforces heterotypic Ping-Pong between Aub and Ago3, ensuring that transposons are silenced and maintaining the integrity of the germline genome.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=22099305&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236501
dc.subjectAnimals
dc.subjectArgonaute Proteins
dc.subjectCell Nucleus
dc.subjectComputational Biology
dc.subjectDNA Damage
dc.subjectDNA Transposable Elements
dc.subjectDrosophila Proteins
dc.subjectDrosophila melanogaster
dc.subjectFemale
dc.subjectFertility
dc.subject*Gene Silencing
dc.subject*Genome, Insect
dc.subjectMembrane Transport Proteins
dc.subjectMutation
dc.subjectOocytes
dc.subjectOvary
dc.subjectPeptide Initiation Factors
dc.subjectProtein Structure, Tertiary
dc.subjectRNA Cleavage
dc.subjectRNA, Small Interfering
dc.subjectRNA-Induced Silencing Complex
dc.subjectSignal Transduction
dc.subjectUbiquitin-Protein Ligases
dc.subjectUMCCTS funding
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.titleHeterotypic piRNA Ping-Pong requires qin, a protein with both E3 ligase and Tudor domains
dc.typeJournal Article
dc.source.journaltitleMolecular cell
dc.source.volume44
dc.source.issue4
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/bmp_pp/155
dc.identifier.contextkey3563843
html.description.abstract<p>piRNAs guide PIWI proteins to silence transposons in animal germ cells. Reciprocal cycles of piRNA-directed RNA cleavage--catalyzed by the PIWI proteins Aubergine (Aub) and Argonaute3 (Ago3) in Drosophila melanogaster--expand the population of antisense piRNAs in response to transposon expression, a process called the Ping-Pong cycle. Heterotypic Ping-Pong between Aub and Ago3 ensures that antisense piRNAs predominate. We show that qin, a piRNA pathway gene whose protein product contains both E3 ligase and Tudor domains, colocalizes with Aub and Ago3 in nuage, a perinuclear structure implicated in transposon silencing. In qin mutants, less Ago3 binds Aub, futile Aub:Aub homotypic Ping-Pong prevails, antisense piRNAs decrease, many families of mobile genetic elements are reactivated, and DNA damage accumulates in nurse cells and oocytes. We propose that Qin enforces heterotypic Ping-Pong between Aub and Ago3, ensuring that transposons are silenced and maintaining the integrity of the germline genome.</p>
dc.identifier.submissionpathbmp_pp/155
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentProgram in Bioinformatics and Integrative Biology
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.source.pages572-84


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