Heterotypic piRNA Ping-Pong requires qin, a protein with both E3 ligase and Tudor domains
dc.contributor.author | Zhang, Zhao | |
dc.contributor.author | Xu, Jia | |
dc.contributor.author | Koppetsch, Birgit S. | |
dc.contributor.author | Wang, Jie | |
dc.contributor.author | Tipping, Cindy | |
dc.contributor.author | Ma, Shengmei | |
dc.contributor.author | Weng, Zhiping | |
dc.contributor.author | Theurkauf, William E. | |
dc.contributor.author | Zamore, Phillip D. | |
dc.date | 2022-08-11T08:08:00.000 | |
dc.date.accessioned | 2022-08-23T15:38:51Z | |
dc.date.available | 2022-08-23T15:38:51Z | |
dc.date.issued | 2011-11-18 | |
dc.date.submitted | 2013-01-04 | |
dc.identifier.citation | <p>Mol Cell. 2011 Nov 18;44(4):572-84. doi: 10.1016/j.molcel.2011.10.011. <a href="http://dx.doi.org/10.1016/j.molcel.2011.10.011">Link to article on publisher's site</a></p> | |
dc.identifier.issn | 1097-2765 (Linking) | |
dc.identifier.doi | 10.1016/j.molcel.2011.10.011 | |
dc.identifier.pmid | 22099305 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/26025 | |
dc.description | <p>Author Zhao Zhang is a student in the Interdisciplinary Graduate Program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.</p> | |
dc.description.abstract | piRNAs guide PIWI proteins to silence transposons in animal germ cells. Reciprocal cycles of piRNA-directed RNA cleavage--catalyzed by the PIWI proteins Aubergine (Aub) and Argonaute3 (Ago3) in Drosophila melanogaster--expand the population of antisense piRNAs in response to transposon expression, a process called the Ping-Pong cycle. Heterotypic Ping-Pong between Aub and Ago3 ensures that antisense piRNAs predominate. We show that qin, a piRNA pathway gene whose protein product contains both E3 ligase and Tudor domains, colocalizes with Aub and Ago3 in nuage, a perinuclear structure implicated in transposon silencing. In qin mutants, less Ago3 binds Aub, futile Aub:Aub homotypic Ping-Pong prevails, antisense piRNAs decrease, many families of mobile genetic elements are reactivated, and DNA damage accumulates in nurse cells and oocytes. We propose that Qin enforces heterotypic Ping-Pong between Aub and Ago3, ensuring that transposons are silenced and maintaining the integrity of the germline genome. | |
dc.language.iso | en_US | |
dc.relation | <p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=22099305&dopt=Abstract">Link to Article in PubMed</a></p> | |
dc.relation.url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236501 | |
dc.subject | Animals | |
dc.subject | Argonaute Proteins | |
dc.subject | Cell Nucleus | |
dc.subject | Computational Biology | |
dc.subject | DNA Damage | |
dc.subject | DNA Transposable Elements | |
dc.subject | Drosophila Proteins | |
dc.subject | Drosophila melanogaster | |
dc.subject | Female | |
dc.subject | Fertility | |
dc.subject | *Gene Silencing | |
dc.subject | *Genome, Insect | |
dc.subject | Membrane Transport Proteins | |
dc.subject | Mutation | |
dc.subject | Oocytes | |
dc.subject | Ovary | |
dc.subject | Peptide Initiation Factors | |
dc.subject | Protein Structure, Tertiary | |
dc.subject | RNA Cleavage | |
dc.subject | RNA, Small Interfering | |
dc.subject | RNA-Induced Silencing Complex | |
dc.subject | Signal Transduction | |
dc.subject | Ubiquitin-Protein Ligases | |
dc.subject | UMCCTS funding | |
dc.subject | Biochemistry, Biophysics, and Structural Biology | |
dc.title | Heterotypic piRNA Ping-Pong requires qin, a protein with both E3 ligase and Tudor domains | |
dc.type | Journal Article | |
dc.source.journaltitle | Molecular cell | |
dc.source.volume | 44 | |
dc.source.issue | 4 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/bmp_pp/155 | |
dc.identifier.contextkey | 3563843 | |
html.description.abstract | <p>piRNAs guide PIWI proteins to silence transposons in animal germ cells. Reciprocal cycles of piRNA-directed RNA cleavage--catalyzed by the PIWI proteins Aubergine (Aub) and Argonaute3 (Ago3) in Drosophila melanogaster--expand the population of antisense piRNAs in response to transposon expression, a process called the Ping-Pong cycle. Heterotypic Ping-Pong between Aub and Ago3 ensures that antisense piRNAs predominate. We show that qin, a piRNA pathway gene whose protein product contains both E3 ligase and Tudor domains, colocalizes with Aub and Ago3 in nuage, a perinuclear structure implicated in transposon silencing. In qin mutants, less Ago3 binds Aub, futile Aub:Aub homotypic Ping-Pong prevails, antisense piRNAs decrease, many families of mobile genetic elements are reactivated, and DNA damage accumulates in nurse cells and oocytes. We propose that Qin enforces heterotypic Ping-Pong between Aub and Ago3, ensuring that transposons are silenced and maintaining the integrity of the germline genome.</p> | |
dc.identifier.submissionpath | bmp_pp/155 | |
dc.contributor.department | Program in Molecular Medicine | |
dc.contributor.department | Program in Bioinformatics and Integrative Biology | |
dc.contributor.department | Department of Biochemistry and Molecular Pharmacology | |
dc.source.pages | 572-84 |