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dc.contributor.authorMcGraw, Bonnie R.
dc.contributor.authorMarinus, Martin G.
dc.date2022-08-11T08:08:00.000
dc.date.accessioned2022-08-23T15:38:52Z
dc.date.available2022-08-23T15:38:52Z
dc.date.issued1980-01-01
dc.date.submitted2009-01-08
dc.identifier.citationMol Gen Genet. 1980;178(2):309-15.
dc.identifier.issn0026-8925 (Print)
dc.identifier.pmid6993844
dc.identifier.urihttp://hdl.handle.net/20.500.14038/26030
dc.description.abstractBacteria mutant in the dam (DNA adenine methylation) gene and in either recA or recB or recC genes are inviable (Virm- phenotype). From crosses between dam-3 bacteria and recA1 or recB21 recC22 strains, Vrm+ recombinants were recovered. Among these recombinants, Dam+ revertants were present which did not show the phenotypes normally associated with dam-3 bacteria. Three classes of indirectly suppressed strains (dam-3 genotype) were also recovered which showed alterations in the secondary phenotypes normally associated with dam-3 bacteria. These strains contained a second unlinked mutation in either mutL or mutS or sin. In addition, mutation in either sbcA or sbcB supresses the Vrm- phenotype of dam-3 recB21 recC22 strains.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=6993844&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1007/BF00270477
dc.subjectAdenine
dc.subjectCrosses, Genetic
dc.subjectDNA (Cytosine-5-)-Methyltransferase
dc.subjectEscherichia coli
dc.subject*Genes
dc.subjectMethyltransferases
dc.subjectMutation
dc.subjectPhenotype
dc.subject*Recombination, Genetic
dc.subject*Suppression, Genetic
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.titleIsolation and characterization of Dam+ revertants and suppressor mutations that modify secondary phenotypes of dam-3 strains of Escherichia coli K-12
dc.typeJournal Article
dc.source.journaltitleMolecular and general genetics : MGG
dc.source.volume178
dc.source.issue2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/bmp_pp/16
dc.identifier.contextkey690842
html.description.abstract<p>Bacteria mutant in the dam (DNA adenine methylation) gene and in either recA or recB or recC genes are inviable (Virm- phenotype). From crosses between dam-3 bacteria and recA1 or recB21 recC22 strains, Vrm+ recombinants were recovered. Among these recombinants, Dam+ revertants were present which did not show the phenotypes normally associated with dam-3 bacteria. Three classes of indirectly suppressed strains (dam-3 genotype) were also recovered which showed alterations in the secondary phenotypes normally associated with dam-3 bacteria. These strains contained a second unlinked mutation in either mutL or mutS or sin. In addition, mutation in either sbcA or sbcB supresses the Vrm- phenotype of dam-3 recB21 recC22 strains.</p>
dc.identifier.submissionpathbmp_pp/16
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.source.pages309-15


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