Argonaute divides its RNA guide into domains with distinct functions and RNA-binding properties
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UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyDocument Type
Journal ArticlePublication Date
2012-11-21Keywords
AnimalsArgonaute Proteins
Base Sequence
Drosophila Proteins
Drosophila melanogaster
Mice
MicroRNAs
*Models, Biological
*RNA Interference
RNA, Guide
RNA, Small Interfering
RNA-Induced Silencing Complex
Biochemistry, Biophysics, and Structural Biology
Genetics and Genomics
Molecular Genetics
Metadata
Show full item recordAbstract
MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) guide Argonaute proteins to silence mRNA expression. Argonaute binding alters the properties of an RNA guide, creating functional domains. We show that the domains established by Argonaute-the anchor, seed, central, 3' supplementary, and tail regions-have distinct biochemical properties that explain the differences between how animal miRNAs and siRNAs bind their targets. Extensive complementarity between an siRNA and its target slows the rate at which fly Argonaute2 (Ago2) binds to and dissociates from the target. Highlighting its role in antiviral defense, fly Ago2 dissociates so slowly from extensively complementary target RNAs that essentially every fully paired target is cleaved. Conversely, mouse AGO2, which mainly mediates miRNA-directed repression, dissociates rapidly and with similar rates for fully paired and seed-matched targets. Our data narrow the range of biochemically reasonable models for how Argonaute-bound siRNAs and miRNAs find, bind, and regulate their targets.Source
Cell. 2012 Nov 21;151(5):1055-67. doi: 10.1016/j.cell.2012.10.036. Link to article on publisher's site
DOI
10.1016/j.cell.2012.10.036Permanent Link to this Item
http://hdl.handle.net/20.500.14038/26031PubMed ID
23178124Notes
Co-author LiangMeng Wee is a student in the Interdisciplinary Graduate Program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.
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10.1016/j.cell.2012.10.036