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    Purification of the receptor for nerve growth factor from A875 melanoma cells by affinity chromatography

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    Authors
    Puma, Patricia
    Buxser, Stephen E.
    Watson, Leslie
    Kelleher, Daniel J.
    Johnson, Gary L.
    UMass Chan Affiliations
    Biochemistry and Molecular Pharmacology
    Neurobiology
    Document Type
    Journal Article
    Publication Date
    1983-03-10
    Keywords
    Cell Line
    Cell Membrane
    Chromatography, Affinity
    Humans
    Kinetics
    Melanoma
    Molecular Weight
    Nerve Growth Factors
    Receptors, Cell Surface
    Receptors, Nerve Growth Factor
    Biochemistry
    Biochemistry, Biophysics, and Structural Biology
    Molecular Biology
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    Link to Full Text
    http://www.jbc.org/content/258/5/3370.full.pdf+html
    Abstract
    The receptor for nerve growth factor (NGF) has been purified to near homogeneity from octylglucoside extracts of A875 melanoma cell membranes by the use of repetitive affinity chromatography on NGF-Sepharose. Elution of purified receptor (NGF receptor) was accomplished with 0.15 M NaCl, pH 11.0, containing phosphatidylcholine and octylglucoside. Chromatography on two columns of NGF-Sepharose yielded a 1500-fold purification of the receptor, as assessed by 125I-NGF binding, and permitted recovery of 9% of the total binding activity in the soluble extract. Scatchard analysis of equilibrium binding of 125I-NGF provided similar Kd values for NGF receptors in soluble extracts of A875 membranes (2.2 nM) and with purified NGF receptor (3.1 nM). Examination of NGF receptor after electrophoresis on sodium dodecyl sulfate-polyacrylamide gels revealed the presence of two major peptides, of Mr = 85,000 and Mr = 200,000. Affinity labeling experiments, done with 125I-NGF and A875 cells, soluble extracts of A875 cell membranes, and purified receptor, show that both of these components of the NGF receptor can be specifically cross-linked to 125I-NGF.
    Source
    J Biol Chem. 1983 Mar 10;258(5):3370-5.
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/26058
    PubMed ID
    6298234
    Related Resources
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    UMass Chan Faculty and Researcher Publications
    Neurobiology Faculty Publications

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