Mutagenesis and repair of DNA damage caused by nitrogen mustard, N,N'-bis(2-chloroethyl)-N-nitrosourea (BCNU), streptozotocin, and mitomycin C in E. coli
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UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyDocument Type
Journal ArticlePublication Date
1986-11-01Keywords
Carmustine*DNA Damage
*DNA Glycosylases
DNA Repair
DNA, Bacterial
Escherichia coli
Genes, Bacterial
Mechlorethamine
Methyltransferases
Mitomycin
Mitomycins
N-Glycosyl Hydrolases
Rec A Recombinases
SOS Response (Genetics)
Site-Specific DNA-Methyltransferase (Adenine-Specific)
Streptozocin
Biochemistry, Biophysics, and Structural Biology
Pharmacology, Toxicology and Environmental Health
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Show full item recordAbstract
Cytotoxicity and mutagenesis by streptozotocin, BCNU, nitrogen mustard, and mitomycin C were evaluated in E. coli mutants deficient in SOS repair, SOS-mediated mutagenesis, the adaptive response, and mutants that engage in aberrant mismatch repair. The results demonstrate that premutagenic lesions are caused by nitrogen mustard, BCNU and streptozotocin that are not repaired by ada or recognized by umuDC. Further, recA mutants were hypomutable after exposure to nitrogen mustard, BCNU, and streptozotocin compared to wild type. With the exception of the monofunctional nitrosourea, streptozotocin, both recA and uvrA gene products contribute to the repair of DNA damage caused by the alkylating agents tested. In the case of streptozotocin, although recA mutants were more sensitive than wild type, uvrA mutants were not. Moreover, while ada and alkA E. coli mutants showed increased sensitivity to streptozotocin, they were not more sensitive to the other alkylating agents evaluated.Source
Mutat Res. 1986 Nov;166(3):299-42.
DOI
10.1016/0167-8817(86)90023-4Permanent Link to this Item
http://hdl.handle.net/20.500.14038/26087PubMed ID
2946949Related Resources
ae974a485f413a2113503eed53cd6c53
10.1016/0167-8817(86)90023-4