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Expression of the Escherichia coli dam gene

dc.contributor.authorLobner-Olesen, Anders
dc.contributor.authorBoye, Erik
dc.contributor.authorMarinus, Martin G.
dc.date2022-08-11T08:08:00.000
dc.date.accessioned2022-08-23T15:39:10Z
dc.date.available2022-08-23T15:39:10Z
dc.date.issued1992-07-01
dc.date.submitted2009-01-12
dc.identifier.citationMol Microbiol. 1992 Jul;6(13):1841-51.
dc.identifier.issn0950-382X (Print)
dc.identifier.pmid1630320
dc.identifier.urihttp://hdl.handle.net/20.500.14038/26101
dc.description.abstractThe Escherichia coli dam gene and upstream sequences were cloned from the Kohara phage 4D4. Five promoters were found to contribute to dam gene transcription. P1 and P2 (the major promoter) were situated approximately 3.5 kb upstream of the structural gene, P3 was within the aroB gene, P4 was within the urf74.3 gene, and P5 was in the urf74.3-dam intergenic region. The nucleotide sequence of 2280 bp of DNA containing P1 and P2 was determined and shown to have the potential to encode a protein of approximately 16 kDa between P1, P2 and the aroB gene. This 16 kDa open reading frame has been identified as aroK, the gene for shikimic acid kinase I. Thus the dam gene is part of an operon containing aroK, aroB, urf74.3, and dam. The transcriptional start points of the promoters were determined. A comparison of their nucleotide sequences suggested that P1-P4 were all recognized by the sigma 70 subunit of the RNA polymerase.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=1630320&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1111/j.1365-2958.1992.tb01356.x
dc.subjectAmino Acid Sequence
dc.subjectBase Sequence
dc.subjectCloning, Molecular
dc.subjectEscherichia coli
dc.subjectEscherichia coli Proteins
dc.subjectFlow Cytometry
dc.subject*Gene Expression Regulation, Bacterial
dc.subjectGenes, Bacterial
dc.subjectMethyltransferases
dc.subjectMolecular Sequence Data
dc.subjectMutation
dc.subjectOperon
dc.subjectPromoter Regions, Genetic
dc.subject*Site-Specific DNA-Methyltransferase (Adenine-Specific)
dc.subjectTranscription Factors
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.subjectPharmacology, Toxicology and Environmental Health
dc.titleExpression of the Escherichia coli dam gene
dc.typeJournal Article
dc.source.journaltitleMolecular microbiology
dc.source.volume6
dc.source.issue13
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/bmp_pp/40
dc.identifier.contextkey692456
html.description.abstract<p>The Escherichia coli dam gene and upstream sequences were cloned from the Kohara phage 4D4. Five promoters were found to contribute to dam gene transcription. P1 and P2 (the major promoter) were situated approximately 3.5 kb upstream of the structural gene, P3 was within the aroB gene, P4 was within the urf74.3 gene, and P5 was in the urf74.3-dam intergenic region. The nucleotide sequence of 2280 bp of DNA containing P1 and P2 was determined and shown to have the potential to encode a protein of approximately 16 kDa between P1, P2 and the aroB gene. This 16 kDa open reading frame has been identified as aroK, the gene for shikimic acid kinase I. Thus the dam gene is part of an operon containing aroK, aroB, urf74.3, and dam. The transcriptional start points of the promoters were determined. A comparison of their nucleotide sequences suggested that P1-P4 were all recognized by the sigma 70 subunit of the RNA polymerase.</p>
dc.identifier.submissionpathbmp_pp/40
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.source.pages1841-51


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