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dc.contributor.authorPalmer, Barry R.
dc.contributor.authorMarinus, Martin G.
dc.date2022-08-11T08:08:00.000
dc.date.accessioned2022-08-23T15:39:11Z
dc.date.available2022-08-23T15:39:11Z
dc.date.issued1994-05-27
dc.date.submitted2009-01-12
dc.identifier.citationGene. 1994 May 27;143(1):1-12.
dc.identifier.issn0378-1119 (Print)
dc.identifier.pmid8200522
dc.identifier.urihttp://hdl.handle.net/20.500.14038/26105
dc.description.abstractThe construction of a variety of strains deficient in the methylation of adenine and cytosine residues in DNA by the methyltransferases (MTases) Dam and Dcm has allowed the study of the role of these enzymes in the biology of Escherichia coli. Dam methylation has been shown to play a role in coordinating DNA replication initiation, DNA mismatch repair and the regulation of expression of some genes. The regulation of expression of dam has been found to be complex and influenced by five promoters. A role for Dcm methylation in the cell remains elusive and dcm- cells have no obvious phenotype. dam- and dcm- strains have a range of uses in molecular biology and bacterial genetics, including preparation of DNA for restriction by some restriction endonucleases, for transformation into other bacterial species, nucleotide sequencing and site-directed mutagenesis. A variety of assays are available for rapid detection of both the Dam and Dcm phenotypes. A number of restriction systems in E. coli have been described which recognise foreign DNA methylation, but ignore Dam and Dcm methylation. Here, we describe the most commonly used mutant alleles of dam and dcm and the characteristics of a variety of the strains that carry these genes. A description of several plasmids that carry dam gene constructs is also included.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=8200522&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1016/0378-1119(94)90597-5
dc.subjectAlleles
dc.subjectCloning, Molecular
dc.subjectDNA (Cytosine-5-)-Methyltransferase
dc.subjectDNA Repair
dc.subjectDNA, Bacterial
dc.subjectEscherichia coli
dc.subjectEscherichia coli Proteins
dc.subjectGene Expression Regulation, Enzymologic
dc.subject*Genes, Bacterial
dc.subjectMethylation
dc.subjectMethyltransferases
dc.subjectMutation
dc.subjectPlasmids
dc.subject*Site-Specific DNA-Methyltransferase (Adenine-Specific)
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.subjectPharmacology, Toxicology and Environmental Health
dc.titleThe dam and dcm strains of Escherichia coli--a review
dc.typeJournal Article
dc.source.journaltitleGene
dc.source.volume143
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/bmp_pp/44
dc.identifier.contextkey692460
html.description.abstract<p>The construction of a variety of strains deficient in the methylation of adenine and cytosine residues in DNA by the methyltransferases (MTases) Dam and Dcm has allowed the study of the role of these enzymes in the biology of Escherichia coli. Dam methylation has been shown to play a role in coordinating DNA replication initiation, DNA mismatch repair and the regulation of expression of some genes. The regulation of expression of dam has been found to be complex and influenced by five promoters. A role for Dcm methylation in the cell remains elusive and dcm- cells have no obvious phenotype. dam- and dcm- strains have a range of uses in molecular biology and bacterial genetics, including preparation of DNA for restriction by some restriction endonucleases, for transformation into other bacterial species, nucleotide sequencing and site-directed mutagenesis. A variety of assays are available for rapid detection of both the Dam and Dcm phenotypes. A number of restriction systems in E. coli have been described which recognise foreign DNA methylation, but ignore Dam and Dcm methylation. Here, we describe the most commonly used mutant alleles of dam and dcm and the characteristics of a variety of the strains that carry these genes. A description of several plasmids that carry dam gene constructs is also included.</p>
dc.identifier.submissionpathbmp_pp/44
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.source.pages1-12


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