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Growth-rate-dependent transcription initiation from the dam P2 promoter

dc.contributor.authorRasmussen, Lene Juel
dc.contributor.authorLobner-Olesen, Anders
dc.contributor.authorMarinus, Martin G.
dc.date2022-08-11T08:08:00.000
dc.date.accessioned2022-08-23T15:39:11Z
dc.date.available2022-08-23T15:39:11Z
dc.date.issued1995-05-19
dc.date.submitted2009-01-12
dc.identifier.citation<p>Gene. 1995 May 19;157(1-2):213-5.</p>
dc.identifier.issn0378-1119 (Print)
dc.identifier.doi10.1016/0378-1119(94)00619-4
dc.identifier.pmid7607492
dc.identifier.urihttp://hdl.handle.net/20.500.14038/26106
dc.description.abstractTranscription of the dam gene in Escherichia coli is dependent on growth rate. Using single-copy promoter::lacZYA fusions we found that of the five promoter regions which affect dam expression, only the P2 promoter shows growth-rate dependence. The determinants for growth-rate control must lie in the region -52 to +27 relative to the transcription start point.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=7607492&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1016/0378-1119(94)00619-4
dc.subjectBase Sequence
dc.subjectColon
dc.subjectEscherichia coli
dc.subjectEscherichia coli Proteins
dc.subject*Gene Expression Regulation, Bacterial
dc.subjectHumans
dc.subjectMethyltransferases
dc.subject*Promoter Regions, Genetic
dc.subjectRecombinant Fusion Proteins
dc.subject*Site-Specific DNA-Methyltransferase (Adenine-Specific)
dc.subjectSubstrate Specificity
dc.subjectbeta-Galactosidase
dc.subjectDNA methylation
dc.subjectlipoic acid ligase
dc.subjectrecombinant DNA
dc.subjectgene regulation
dc.subjectBacteria
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.subjectGenetic Phenomena
dc.subjectPharmacology, Toxicology and Environmental Health
dc.titleGrowth-rate-dependent transcription initiation from the dam P2 promoter
dc.typeJournal Article
dc.source.journaltitleGene
dc.source.volume157
dc.source.issue1-2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/bmp_pp/45
dc.identifier.contextkey692461
html.description.abstract<p>Transcription of the dam gene in Escherichia coli is dependent on growth rate. Using single-copy promoter::lacZYA fusions we found that of the five promoter regions which affect dam expression, only the P2 promoter shows growth-rate dependence. The determinants for growth-rate control must lie in the region -52 to +27 relative to the transcription start point.</p>
dc.identifier.submissionpathbmp_pp/45
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.source.pages213-5


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