Glycolipids of the mouse peritoneal macrophage. Alterations in amount and surface exposure of specific glycolipid species occur in response to inflammation and tumoricidal activation
Document Type
Journal ArticlePublication Date
1984-10-01Keywords
Animals*Antigens, CD
Ascitic Fluid
Female
G(M1) Ganglioside
Galactosylceramides
Glycolipids
Glycosphingolipids
Inflammation
*Lactosylceramides
Leukemia, Experimental
*Macrophage Activation
Macrophages
Membrane Lipids
Mice
Mice, Inbred C57BL
Cancer Biology
Cell Biology
Neoplasms
Metadata
Show full item recordAbstract
We have characterized the major glycolipid constituents of the mouse peritoneal macrophage, and have demonstrated that alterations in the amount and in the accessibility of specific glycolipid species to galactose oxidase/NaB3H4 labeling, an indicator of glycolipid surface exposure, occur in response to inflammation and as a consequence of activation to a tumoricidal state. The key findings are: (a) Asialo GM1, a major neutral glycolipid constituent of all macrophage populations examined, is accessible to galactose oxidase/NaB3H4 labeling on the surface of TG-elicited and BCG-activated macrophages but not on resident macrophages; (b) GM1 is the predominant ganglioside constituent of the mouse macrophage. Resident macrophages contain two distinct GM1 species, as determined by cholera toxin binding, while TG-elicited and BCG-activated macrophages contain an additional GM1 species. Differences in the relative amounts of these GM1 species, as well as in their accessibility to galactose oxidase/NaB3H4 labeling, exist among the macrophage populations. These observations suggest that both a chemical and spatial reorganization of surface glycolipids occurs in response to inflammation and tumoricidal activation.Source
J Exp Med. 1984 Oct 1;160(4):1114-25. Link to article on publisher's websiteDOI
10.1084/jem.160.4.1114Permanent Link to this Item
http://hdl.handle.net/20.500.14038/26196PubMed ID
6481302Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1084/jem.160.4.1114