Functional analysis of the contribution of RhoA and RhoC GTPases to invasive breast carcinoma
UMass Chan Affiliations
Department of Cancer BiologyDocument Type
Journal ArticlePublication Date
2004-12-03Keywords
ADP Ribose TransferasesBreast Neoplasms
Cell Adhesion
Cell Line, Tumor
Humans
Neoplasm Invasiveness
RNA Interference
RNA, Small Interfering
Signal Transduction
rho GTP-Binding Proteins
rhoA GTP-Binding Protein
Cancer Biology
Neoplasms
Metadata
Show full item recordAbstract
Although the RhoA and RhoC proteins comprise an important subset of the Rho GTPase family that have been implicated in invasive breast carcinomas, attributing specific functions to these individual members has been difficult. We have used a stable retroviral RNA interference approach to generate invasive breast carcinoma cells (SUM-159 cells) that lack either RhoA or RhoC expression. Analysis of these cells enabled us to deduce that RhoA impedes and RhoC stimulates invasion. Unexpectedly, this analysis also revealed a compensatory relationship between RhoA and RhoC at the level of both their expression and activation, and a reciprocal relationship between RhoA and Rac1 activation.Source
Cancer Res. 2004 Dec 1;64(23):8694-701. Link to article on publisher's siteDOI
10.1158/0008-5472.CAN-04-2247Permanent Link to this Item
http://hdl.handle.net/20.500.14038/26263PubMed ID
15574779Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1158/0008-5472.CAN-04-2247