Show simple item record

dc.contributor.authorWilkerson, Curtis G.
dc.contributor.authorKing, Stephen M.
dc.contributor.authorKoutoulis, Anthony
dc.contributor.authorPazour, Gregory J.
dc.contributor.authorWitman, George B.
dc.date2022-08-11T08:08:03.000
dc.date.accessioned2022-08-23T15:40:31Z
dc.date.available2022-08-23T15:40:31Z
dc.date.issued1995-04-01
dc.date.submitted2008-12-11
dc.identifier.citation<p>J Cell Biol. 1995 Apr;129(1):169-78.</p>
dc.identifier.issn0021-9525 (Print)
dc.identifier.doi10.1083/jcb.129.1.169
dc.identifier.pmid7698982
dc.identifier.urihttp://hdl.handle.net/20.500.14038/26418
dc.description.abstractWe have isolated and sequenced a full-length cDNA clone encoding the 78,000 Mr intermediate chain (IC78) of the Chlamydomonas outer arm dynein. This protein previously was shown to be located at the base of the solubilized dynein particle and to interact with alpha tubulin in situ, suggesting that it may be involved in binding the outer arm to the doublet microtubule. The sequence predicts a polypeptide of 683 amino acids having a mass of 76.5 kD. Sequence comparison indicates that IC78 is homologous to the 69,000 M(r) intermediate chain (IC69) of Chlamydomonas outer arm dynein and to the 74,000 M(r) intermediate chain (IC74) of cytoplasmic dynein. The similarity between the chains is greatest in their COOH-terminal halves; the NH(2)-terminal halves are highly divergent. The COOH-terminal half of IC78 contains six short imperfect repeats, termed WD repeats, that are thought to be involved in protein-protein interactions. Although not previously reported, these repeated elements also are present in IC69 and IC74. Using the IC78 cDNA as a probe, we screened a group of slow-swimming insertional mutants and identified one which has a large insertion in the IC78 gene and seven in which the IC78 gene is completely deleted. Electron microscopy of three of these IC78 mutants revealed that each is missing the outer arm, indicating that IC78 is essential for arm assembly or attachment to the outer doublet. Restriction fragment length polymorphism mapping places the IC78 gene on the left arm of chromosome XII/XIII, at or near the mutation oda9, which also causes loss of the outer arm. Mutants with defects in the IC78 gene do not complement the oda9 mutation in stable diploids, strongly suggesting that ODA9 is the structural gene for IC78.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=7698982&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120364
dc.subjectAmino Acid Sequence
dc.subjectAnimals
dc.subjectBase Sequence
dc.subjectBlotting, Southern
dc.subjectChlamydomonas reinhardtii
dc.subjectConsensus Sequence
dc.subjectDynein ATPase
dc.subjectFlagella
dc.subjectMacromolecular Substances
dc.subjectMicrotubules
dc.subjectMolecular Sequence Data
dc.subjectMolecular Weight
dc.subjectMutagenesis, Insertional
dc.subjectOligonucleotide Probes
dc.subjectProtein Biosynthesis
dc.subjectRecombinant Proteins
dc.subject*Repetitive Sequences, Nucleic Acid
dc.subjectSequence Homology, Amino Acid
dc.subjectTranscription, Genetic
dc.subjectAmino Acids, Peptides, and Proteins
dc.subjectCell Biology
dc.subjectGenetic Phenomena
dc.titleThe 78,000 M(r) intermediate chain of Chlamydomonas outer arm dynein isa WD-repeat protein required for arm assembly
dc.typeJournal Article
dc.source.journaltitleThe Journal of cell biology
dc.source.volume129
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/cellbiology_pp/10
dc.identifier.contextkey680152
html.description.abstract<p>We have isolated and sequenced a full-length cDNA clone encoding the 78,000 Mr intermediate chain (IC78) of the Chlamydomonas outer arm dynein. This protein previously was shown to be located at the base of the solubilized dynein particle and to interact with alpha tubulin in situ, suggesting that it may be involved in binding the outer arm to the doublet microtubule. The sequence predicts a polypeptide of 683 amino acids having a mass of 76.5 kD. Sequence comparison indicates that IC78 is homologous to the 69,000 M(r) intermediate chain (IC69) of Chlamydomonas outer arm dynein and to the 74,000 M(r) intermediate chain (IC74) of cytoplasmic dynein. The similarity between the chains is greatest in their COOH-terminal halves; the NH(2)-terminal halves are highly divergent. The COOH-terminal half of IC78 contains six short imperfect repeats, termed WD repeats, that are thought to be involved in protein-protein interactions. Although not previously reported, these repeated elements also are present in IC69 and IC74. Using the IC78 cDNA as a probe, we screened a group of slow-swimming insertional mutants and identified one which has a large insertion in the IC78 gene and seven in which the IC78 gene is completely deleted. Electron microscopy of three of these IC78 mutants revealed that each is missing the outer arm, indicating that IC78 is essential for arm assembly or attachment to the outer doublet. Restriction fragment length polymorphism mapping places the IC78 gene on the left arm of chromosome XII/XIII, at or near the mutation oda9, which also causes loss of the outer arm. Mutants with defects in the IC78 gene do not complement the oda9 mutation in stable diploids, strongly suggesting that ODA9 is the structural gene for IC78.</p>
dc.identifier.submissionpathcellbiology_pp/10
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages169-78


This item appears in the following Collection(s)

Show simple item record