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    Interspecies conservation of outer arm dynein intermediate chain sequences defines two intermediate chain subclasses

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    Authors
    Ogawa, Kazuo
    Kamiya, Ritsu
    Wilkerson, Curtis G.
    Witman, George B.
    UMass Chan Affiliations
    Department of Cell Biology
    Document Type
    Journal Article
    Publication Date
    1995-06-01
    Keywords
    Amino Acid Sequence
    Animals
    Base Sequence
    Blotting, Western
    Chlamydomonas
    Cilia
    Cloning, Molecular
    Conserved Sequence
    DNA, Complementary
    Dynein ATPase
    Flagella
    Molecular Sequence Data
    Sea Urchins
    Sequence Alignment
    Sequence Homology, Amino Acid
    Amino Acids, Peptides, and Proteins
    Cell Biology
    Enzymes and Coenzymes
    Investigative Techniques
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    Link to Full Text
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC301229
    Abstract
    Immunological analysis showed that antibodies against the intermediate chains (ICs) IC2 and IC3 of sea urchin outer arm dynein specifically cross-reacted with intermediate chains IC78 and IC69, respectively, of Chlamydomonas outer arm dynein. In contrast, no specific cross-reactivity with any Chlamydomonas outer arm polypeptide was observed using antibody against IC1 of sea urchin outer arm dynein. To learn more about the relationships between the different ICs, overlapping cDNAs encoding all of IC2 and IC3 of sea urchin were isolated and sequenced. Comparison of these sequences with those previously obtained for the Chlamydomonas ICs revealed that, although all four chains are homologous, sea urchin IC2 is much more closely related to Chlamydomonas IC78 (45.8% identity), and sea urchin IC3 is much more closely related to Chlamydomonas IC69 (48.5% identity), than either sea urchin chain is related to the other (23.5% identity). For homologous pairs, the similarities extend throughout the full lengths of the chains. Regions of similarity between all four ICs and the IC (IC74) of cytoplasmic dynein, located in the C-terminal halves of the chains, are due primarily to conservation of the WD repeats present in all of these ICs. This is the first demonstration that structural differences between individual ICs within an outer arm dynein have been highly conserved in the dyneins of distantly related species. The results provide a basis for the subclassification of these chains.
    Source

    Mol Biol Cell. 1995 Jun;6(6):685-96.

    DOI
    10.1091/mbc.6.6.685
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/26429
    PubMed ID
    7579688
    Related Resources

    Link to Article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.1091/mbc.6.6.685
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