Show simple item record

dc.contributor.authorOgawa, Kazuo
dc.contributor.authorKamiya, Ritsu
dc.contributor.authorWilkerson, Curtis G.
dc.contributor.authorWitman, George B.
dc.date2022-08-11T08:08:03.000
dc.date.accessioned2022-08-23T15:40:34Z
dc.date.available2022-08-23T15:40:34Z
dc.date.issued1995-06-01
dc.date.submitted2008-12-11
dc.identifier.citation<p>Mol Biol Cell. 1995 Jun;6(6):685-96.</p>
dc.identifier.issn1059-1524 (Print)
dc.identifier.doi10.1091/mbc.6.6.685
dc.identifier.pmid7579688
dc.identifier.urihttp://hdl.handle.net/20.500.14038/26429
dc.description.abstractImmunological analysis showed that antibodies against the intermediate chains (ICs) IC2 and IC3 of sea urchin outer arm dynein specifically cross-reacted with intermediate chains IC78 and IC69, respectively, of Chlamydomonas outer arm dynein. In contrast, no specific cross-reactivity with any Chlamydomonas outer arm polypeptide was observed using antibody against IC1 of sea urchin outer arm dynein. To learn more about the relationships between the different ICs, overlapping cDNAs encoding all of IC2 and IC3 of sea urchin were isolated and sequenced. Comparison of these sequences with those previously obtained for the Chlamydomonas ICs revealed that, although all four chains are homologous, sea urchin IC2 is much more closely related to Chlamydomonas IC78 (45.8% identity), and sea urchin IC3 is much more closely related to Chlamydomonas IC69 (48.5% identity), than either sea urchin chain is related to the other (23.5% identity). For homologous pairs, the similarities extend throughout the full lengths of the chains. Regions of similarity between all four ICs and the IC (IC74) of cytoplasmic dynein, located in the C-terminal halves of the chains, are due primarily to conservation of the WD repeats present in all of these ICs. This is the first demonstration that structural differences between individual ICs within an outer arm dynein have been highly conserved in the dyneins of distantly related species. The results provide a basis for the subclassification of these chains.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=7579688&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC301229
dc.subjectAmino Acid Sequence
dc.subjectAnimals
dc.subjectBase Sequence
dc.subjectBlotting, Western
dc.subjectChlamydomonas
dc.subjectCilia
dc.subjectCloning, Molecular
dc.subjectConserved Sequence
dc.subjectDNA, Complementary
dc.subjectDynein ATPase
dc.subjectFlagella
dc.subjectMolecular Sequence Data
dc.subjectSea Urchins
dc.subjectSequence Alignment
dc.subjectSequence Homology, Amino Acid
dc.subjectAmino Acids, Peptides, and Proteins
dc.subjectCell Biology
dc.subjectEnzymes and Coenzymes
dc.subjectInvestigative Techniques
dc.titleInterspecies conservation of outer arm dynein intermediate chain sequences defines two intermediate chain subclasses
dc.typeJournal Article
dc.source.journaltitleMolecular biology of the cell
dc.source.volume6
dc.source.issue6
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/cellbiology_pp/11
dc.identifier.contextkey680153
html.description.abstract<p>Immunological analysis showed that antibodies against the intermediate chains (ICs) IC2 and IC3 of sea urchin outer arm dynein specifically cross-reacted with intermediate chains IC78 and IC69, respectively, of Chlamydomonas outer arm dynein. In contrast, no specific cross-reactivity with any Chlamydomonas outer arm polypeptide was observed using antibody against IC1 of sea urchin outer arm dynein. To learn more about the relationships between the different ICs, overlapping cDNAs encoding all of IC2 and IC3 of sea urchin were isolated and sequenced. Comparison of these sequences with those previously obtained for the Chlamydomonas ICs revealed that, although all four chains are homologous, sea urchin IC2 is much more closely related to Chlamydomonas IC78 (45.8% identity), and sea urchin IC3 is much more closely related to Chlamydomonas IC69 (48.5% identity), than either sea urchin chain is related to the other (23.5% identity). For homologous pairs, the similarities extend throughout the full lengths of the chains. Regions of similarity between all four ICs and the IC (IC74) of cytoplasmic dynein, located in the C-terminal halves of the chains, are due primarily to conservation of the WD repeats present in all of these ICs. This is the first demonstration that structural differences between individual ICs within an outer arm dynein have been highly conserved in the dyneins of distantly related species. The results provide a basis for the subclassification of these chains.</p>
dc.identifier.submissionpathcellbiology_pp/11
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages685-96


This item appears in the following Collection(s)

Show simple item record