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dc.contributor.authorByron, Meg
dc.contributor.authorHall, Lisa L.
dc.contributor.authorLawrence, Jeanne B.
dc.date2022-08-11T08:08:03.000
dc.date.accessioned2022-08-23T15:40:35Z
dc.date.available2022-08-23T15:40:35Z
dc.date.issued2013-01-01
dc.date.submitted2013-03-22
dc.identifier.citation<p>Curr Protoc Hum Genet. 2013 Jan;Chapter 4:Unit 4.15. doi: 10.1002/0471142905.hg0415s76. <a href="http://dx.doi.org/10.1002/0471142905.hg0415s76">Link to article on publisher's site</a></p>
dc.identifier.issn1934-8258 (Linking)
dc.identifier.doi10.1002/0471142905.hg0415s76
dc.identifier.pmid23315927
dc.identifier.urihttp://hdl.handle.net/20.500.14038/26435
dc.description.abstractFluorescence in situ hybridization (FISH) is not a singular technique, but a battery of powerful and versatile tools for examining the distribution of endogenous genes and RNAs in precise context with each other and in relation to specific proteins or cell structures. This unit offers the details of highly sensitive and successful protocols that were initially developed largely in our lab and honed over a number of years. Our emphasis is on analysis of nuclear RNAs and DNA to address specific biological questions about nuclear structure, pre-mRNA metabolism, or the role of noncoding RNAs; however, cytoplasmic RNA detection is also discussed. Multifaceted molecular cytological approaches bring precise resolution and sensitive multicolor detection to illuminate the organization and functional roles of endogenous genes and their RNAs within the native structure of fixed cells. Solutions to several common technical pitfalls are discussed, as are cautions regarding the judicious use of digital imaging and the rigors of analyzing and interpreting complex molecular cytological results.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=23315927&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3556644/
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectFISH
dc.subjectfluorescence in situ hybridization
dc.subjectnuclear structure
dc.subjectDNA
dc.subjectRNA
dc.subjectimmunofluorescence
dc.subjectcytogenetics
dc.subjecthistochemistry
dc.subjectchromosomes
dc.subjectCell Biology
dc.subjectCells
dc.subjectGenetic Phenomena
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectLaboratory and Basic Science Research
dc.subjectNucleic Acids, Nucleotides, and Nucleosides
dc.titleA multifaceted FISH approach to study endogenous RNAs and DNAs in native nuclear and cell structures
dc.typeJournal Article
dc.source.journaltitleCurrent protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]
dc.source.volumeChapter 4
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/cellbiology_pp/117
dc.identifier.contextkey3943378
html.description.abstract<p>Fluorescence in situ hybridization (FISH) is not a singular technique, but a battery of powerful and versatile tools for examining the distribution of endogenous genes and RNAs in precise context with each other and in relation to specific proteins or cell structures. This unit offers the details of highly sensitive and successful protocols that were initially developed largely in our lab and honed over a number of years. Our emphasis is on analysis of nuclear RNAs and DNA to address specific biological questions about nuclear structure, pre-mRNA metabolism, or the role of noncoding RNAs; however, cytoplasmic RNA detection is also discussed. Multifaceted molecular cytological approaches bring precise resolution and sensitive multicolor detection to illuminate the organization and functional roles of endogenous genes and their RNAs within the native structure of fixed cells. Solutions to several common technical pitfalls are discussed, as are cautions regarding the judicious use of digital imaging and the rigors of analyzing and interpreting complex molecular cytological results.</p>
dc.identifier.submissionpathcellbiology_pp/117
dc.contributor.departmentDepartment of Cell and Developmental Biology
dc.source.pagesUnit 4.15


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