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    A fast and sensitive alternative for beta-galactosidase detection in mouse embryos

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    Authors
    Sundararajan, Sakthi
    Wakamiya, Maki
    Behringer, Richard R.
    Rivera-Perez, Jaime A.
    UMass Chan Affiliations
    Department of Cell and Developmental Biology
    Document Type
    Journal Article
    Publication Date
    2012-12-01
    Keywords
    Animals
    Galactosides
    Gene Expression Regulation, Developmental
    Genes, Reporter
    Indoles
    *Lac Operon
    Mice
    *Staining and Labeling
    beta-Galactosidase
    Cell and Developmental Biology
    Genetics and Genomics
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    Link to Full Text
    http://dx.doi.org/10.1242/dev.078790
    Abstract
    The bacterial lacZ gene is widely used as a reporter in a myriad of mouse transgenic experiments. beta-Galactosidase, encoded by lacZ, is usually detected using X-gal in combination with ferric and ferrous ions. This assay produces a blue indole precipitate that is easy to detect visually. Here, we show that Salmon-gal in combination with tetrazolium salts provides a more sensitive and faster staining reaction than the traditional beta-galactosidase assay in mouse embryos. Using a combination of Salmon-gal and tetranitroblue tetrazolium, we were able to visualize the activity of beta-galactosidase in embryos at stages when the customary X-gal reaction failed to detect staining. Our studies provide an enhanced alternative for beta-galactosidase detection in expression and cell fate studies that use lacZ-based transgenic mouse lines.
    Source
    Development. 2012 Dec 1;139(23):4484-90. doi: 10.1242/dev.078790. Link to article on publisher's site
    DOI
    10.1242/dev.078790
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/26439
    PubMed ID
    23132248
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1242/dev.078790
    Scopus Count
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    UMass Chan Faculty and Researcher Publications

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