Show simple item record

dc.contributor.authorSundararajan, Sakthi
dc.contributor.authorWakamiya, Maki
dc.contributor.authorBehringer, Richard R.
dc.contributor.authorRivera-Perez, Jaime A.
dc.date2022-08-11T08:08:03.000
dc.date.accessioned2022-08-23T15:40:36Z
dc.date.available2022-08-23T15:40:36Z
dc.date.issued2012-12-01
dc.date.submitted2013-03-22
dc.identifier.citationDevelopment. 2012 Dec 1;139(23):4484-90. doi: 10.1242/dev.078790. <a href="http://dx.doi.org/10.1242/dev.078790">Link to article on publisher's site</a>
dc.identifier.issn0950-1991 (Linking)
dc.identifier.doi10.1242/dev.078790
dc.identifier.pmid23132248
dc.identifier.urihttp://hdl.handle.net/20.500.14038/26439
dc.description.abstractThe bacterial lacZ gene is widely used as a reporter in a myriad of mouse transgenic experiments. beta-Galactosidase, encoded by lacZ, is usually detected using X-gal in combination with ferric and ferrous ions. This assay produces a blue indole precipitate that is easy to detect visually. Here, we show that Salmon-gal in combination with tetrazolium salts provides a more sensitive and faster staining reaction than the traditional beta-galactosidase assay in mouse embryos. Using a combination of Salmon-gal and tetranitroblue tetrazolium, we were able to visualize the activity of beta-galactosidase in embryos at stages when the customary X-gal reaction failed to detect staining. Our studies provide an enhanced alternative for beta-galactosidase detection in expression and cell fate studies that use lacZ-based transgenic mouse lines.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=23132248&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1242/dev.078790
dc.subjectAnimals
dc.subjectGalactosides
dc.subjectGene Expression Regulation, Developmental
dc.subjectGenes, Reporter
dc.subjectIndoles
dc.subject*Lac Operon
dc.subjectMice
dc.subject*Staining and Labeling
dc.subjectbeta-Galactosidase
dc.subjectCell and Developmental Biology
dc.subjectGenetics and Genomics
dc.titleA fast and sensitive alternative for beta-galactosidase detection in mouse embryos
dc.typeJournal Article
dc.source.journaltitleDevelopment (Cambridge, England)
dc.source.volume139
dc.source.issue23
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/cellbiology_pp/121
dc.identifier.contextkey3943382
html.description.abstract<p>The bacterial lacZ gene is widely used as a reporter in a myriad of mouse transgenic experiments. beta-Galactosidase, encoded by lacZ, is usually detected using X-gal in combination with ferric and ferrous ions. This assay produces a blue indole precipitate that is easy to detect visually. Here, we show that Salmon-gal in combination with tetrazolium salts provides a more sensitive and faster staining reaction than the traditional beta-galactosidase assay in mouse embryos. Using a combination of Salmon-gal and tetranitroblue tetrazolium, we were able to visualize the activity of beta-galactosidase in embryos at stages when the customary X-gal reaction failed to detect staining. Our studies provide an enhanced alternative for beta-galactosidase detection in expression and cell fate studies that use lacZ-based transgenic mouse lines.</p>
dc.identifier.submissionpathcellbiology_pp/121
dc.contributor.departmentDepartment of Cell and Developmental Biology
dc.source.pages4484-90


This item appears in the following Collection(s)

Show simple item record