Direct force probe reveals the mechanics of nuclear homeostasis in the mammalian cell
Chancellor, T J.
Nickerson, Jeffrey A.
Roux, Kyle J.
Dickinson, Richard B.
Lele, Tanmay P.
UMass Chan AffiliationsDepartment of Cell and Developmental Biology
Cell Line, Tumor
*Gene Expression Regulation
Green Fluorescent Proteins
NIH 3T3 Cells
RNA, Small Interfering
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AbstractHow cells maintain nuclear shape and position against various intracellular and extracellular forces is not well understood, although defects in nuclear mechanical homeostasis are associated with a variety of human diseases. We estimated the force required to displace and deform the nucleus in adherent living cells with a technique to locally pull the nuclear surface. A minimum pulling force of a few nanonewtons--far greater than typical intracellular motor forces--was required to significantly displace and deform the nucleus. Upon force removal, the original shape and position were restored quickly within a few seconds. This stiff, elastic response required the presence of vimentin, lamin A/C, and SUN (Sad1p, UNC-84)-domain protein linkages, but not F-actin or microtubules. Although F-actin and microtubules are known to exert mechanical forces on the nuclear surface through molecular motor activity, we conclude that the intermediate filament networks maintain nuclear mechanical homeostasis against localized forces.
SourceProc Natl Acad Sci U S A. 2015 May 5;112(18):5720-5. doi: 10.1073/pnas.1502111112. Epub 2015 Apr 21. Link to article on publisher's site.
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/26489
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