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dc.contributor.authorZhang, Qiao
dc.contributor.authorKota, Krishna P.
dc.contributor.authorAlam, Samer G.
dc.contributor.authorNickerson, Jeffrey A.
dc.contributor.authorDickinson, Richard B.
dc.contributor.authorLele, Tanmay P.
dc.date2022-08-11T08:08:03.000
dc.date.accessioned2022-08-23T15:40:50Z
dc.date.available2022-08-23T15:40:50Z
dc.date.issued2016-06-01
dc.date.submitted2016-05-31
dc.identifier.citationJ Cell Physiol. 2016 Jun;231(6):1269-75. doi: 10.1002/jcp.25224. Epub 2015 Nov 24. <a href="http://dx.doi.org/10.1002/jcp.25224">Link to article on publisher's site</a>
dc.identifier.issn0021-9541 (Linking)
dc.identifier.doi10.1002/jcp.25224
dc.identifier.pmid26496460
dc.identifier.urihttp://hdl.handle.net/20.500.14038/26491
dc.description.abstractDespite being densely packed with chromatin, nuclear bodies and a nucleoskeletal network, the nucleus is a remarkably dynamic organelle. Chromatin loops form and relax, RNA transcripts and transcription factors move diffusively, and nuclear bodies move. We show here that RNA splicing speckled domains (splicing speckles) fluctuate in constrained nuclear volumes and remodel their shapes. Small speckles move in a directed way toward larger speckles with which they fuse. This directed movement is reduced upon decreasing cellular ATP levels or inhibiting RNA polymerase II activity. The random movement of speckles is reduced upon decreasing cellular ATP levels, moderately reduced after inhibition of SWI/SNF chromatin remodeling and modestly increased upon inhibiting RNA polymerase II activity. To define the paths through which speckles can translocate in the nucleus, we generated a pressure gradient to create flows in the nucleus. In response to the pressure gradient, speckles moved along curvilinear paths in the nucleus. Collectively, our results demonstrate a new type of ATP-dependent motion in the nucleus. We present a model where recycling splicing factors return as part of small sub-speckles from distal sites of RNA processing to larger splicing speckles by a directed ATP-driven mechanism through interchromatin spaces.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=26496460&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1002/jcp.25224
dc.subjectCell Biology
dc.subjectCellular and Molecular Physiology
dc.titleCoordinated Dynamics of RNA Splicing Speckles in the Nucleus
dc.typeJournal Article
dc.source.journaltitleJournal of cellular physiology
dc.source.volume231
dc.source.issue6
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/cellbiology_pp/175
dc.identifier.contextkey8667026
html.description.abstract<p>Despite being densely packed with chromatin, nuclear bodies and a nucleoskeletal network, the nucleus is a remarkably dynamic organelle. Chromatin loops form and relax, RNA transcripts and transcription factors move diffusively, and nuclear bodies move. We show here that RNA splicing speckled domains (splicing speckles) fluctuate in constrained nuclear volumes and remodel their shapes. Small speckles move in a directed way toward larger speckles with which they fuse. This directed movement is reduced upon decreasing cellular ATP levels or inhibiting RNA polymerase II activity. The random movement of speckles is reduced upon decreasing cellular ATP levels, moderately reduced after inhibition of SWI/SNF chromatin remodeling and modestly increased upon inhibiting RNA polymerase II activity. To define the paths through which speckles can translocate in the nucleus, we generated a pressure gradient to create flows in the nucleus. In response to the pressure gradient, speckles moved along curvilinear paths in the nucleus. Collectively, our results demonstrate a new type of ATP-dependent motion in the nucleus. We present a model where recycling splicing factors return as part of small sub-speckles from distal sites of RNA processing to larger splicing speckles by a directed ATP-driven mechanism through interchromatin spaces.</p>
dc.identifier.submissionpathcellbiology_pp/175
dc.contributor.departmentDepartment of Cell and Developmental Biology
dc.source.pages1269-75


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