The Chlamydomonas reinhardtii ODA3 gene encodes a protein of the outer dynein arm docking complex
Authors
Koutoulis, AnthonyPazour, Gregory J.
Wilkerson, Curtis G.
Inaba, Kazuo
Sheng, Hong
Takada, Saeko
Witman, George B.
Document Type
Journal ArticlePublication Date
1997-06-02Keywords
Amino Acid SequenceAnimals
Base Sequence
Chlamydomonas reinhardtii
Cloning, Molecular
DNA, Complementary
DNA, Plant
Dynein ATPase
Flagella
Genes, Plant
Molecular Sequence Data
Mutagenesis, Insertional
Sequence Analysis, DNA
Amino Acids, Peptides, and Proteins
Cell Biology
Genetic Phenomena
Metadata
Show full item recordAbstract
We have used an insertional mutagenesis/ gene tagging technique to generate new Chlamydomonas reinhardtii mutants that are defective in assembly of the uter ynein rm. Among 39 insertional oda mutants characterized, two are alleles of the previously uncloned ODA3 gene, one is an allele of the uncloned ODA10 gene, and one represents a novel ODA gene (termed ODA12). ODA3 is of particular interest because it is essential for assembly of both the outer dynein arm and the outer dynein arm docking complex (ODA-DC) onto flagellar doublet microtubules (Takada, S., and R. Kamiya. 1994. J. Cell Biol. 126:737- 745). Beginning with the inserted DNA as a tag, the ODA3 gene and a full-length cDNA were cloned. The cloned gene rescues the phenotype of oda3 mutants. The cDNA sequence predicts a novel 83. 4-kD protein with extensive coiled-coil domains. The ODA-DC contains three polypeptides; direct amino acid sequencing indicates that the largest of these polypeptides corresponds to ODA3. This protein is likely to have an important role in the precise positioning of the outer dynein arms on the flagellar axoneme.Source
J Cell Biol. 1997 Jun 2;137(5):1069-80.
DOI
10.1083/jcb.137.5.1069Permanent Link to this Item
http://hdl.handle.net/20.500.14038/26503PubMed ID
9166407Related Resources
ae974a485f413a2113503eed53cd6c53
10.1083/jcb.137.5.1069