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Directionality of brain microtubule assembly in vitro

dc.contributor.authorDentler, William L.
dc.contributor.authorGranett, Sandra
dc.contributor.authorWitman, George B.
dc.contributor.authorRosenbaum, Joel L.
dc.date2022-08-11T08:08:04.000
dc.date.accessioned2022-08-23T15:41:03Z
dc.date.available2022-08-23T15:41:03Z
dc.date.issued1974-05-01
dc.date.submitted2008-12-15
dc.identifier.citation<p>Proc Natl Acad Sci U S A. 1974 May;71(5):1710-4.</p>
dc.identifier.issn0027-8424 (Print)
dc.identifier.doi10.1073/pnas.71.5.1710
dc.identifier.pmid4134974
dc.identifier.urihttp://hdl.handle.net/20.500.14038/26538
dc.description.abstractChicks were injected intra-cerebrally with [(3)H]leucine, and (3)H-labeled microtubules were assembled in vitro in the brain supernatants. Pieces of these (3)H-labeled microtubules were then incubated with unlabeled brain tubulin subunits under conditions where the subunits assembled onto the labeled pieces. Electron microscopic autoradiography of the negatively-stained microtubules showed all of the radioactivity at one end of the tubules as they increased in length. This clearly demonstrated that the microtubules of brain in this in vitro system were being assembled unidirectionally.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=4134974&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC388308
dc.subjectAnimals
dc.subjectAutoradiography
dc.subjectBrain
dc.subjectBrain Chemistry
dc.subjectChickens
dc.subjectElectrophoresis, Polyacrylamide Gel
dc.subjectLeucine
dc.subjectMicroscopy, Electron
dc.subjectMicrotubules
dc.subjectStaining and Labeling
dc.subjectTritium
dc.subjectViscosity
dc.subjectAnimal Experimentation and Research
dc.subjectCell Biology
dc.subjectInvestigative Techniques
dc.subjectNervous System
dc.titleDirectionality of brain microtubule assembly in vitro
dc.typeJournal Article
dc.source.journaltitleProceedings of the National Academy of Sciences of the United States of America
dc.source.volume71
dc.source.issue5
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/cellbiology_pp/42
dc.identifier.contextkey682195
html.description.abstract<p>Chicks were injected intra-cerebrally with [(3)H]leucine, and (3)H-labeled microtubules were assembled in vitro in the brain supernatants. Pieces of these (3)H-labeled microtubules were then incubated with unlabeled brain tubulin subunits under conditions where the subunits assembled onto the labeled pieces. Electron microscopic autoradiography of the negatively-stained microtubules showed all of the radioactivity at one end of the tubules as they increased in length. This clearly demonstrated that the microtubules of brain in this in vitro system were being assembled unidirectionally.</p>
dc.identifier.submissionpathcellbiology_pp/42
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages1710-4


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