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Tubulin requires tau for growth onto microtubule initiating sites

dc.contributor.authorWitman, George B.
dc.contributor.authorCleveland, Don W.
dc.contributor.authorWeingarten, Murray D.
dc.contributor.authorKirschner, Marc W.
dc.date2022-08-11T08:08:04.000
dc.date.accessioned2022-08-23T15:41:03Z
dc.date.available2022-08-23T15:41:03Z
dc.date.issued1976-11-01
dc.date.submitted2008-12-15
dc.identifier.citation<p>Proc Natl Acad Sci U S A. 1976 Nov;73(11):4070-4.</p>
dc.identifier.issn0027-8424 (Print)
dc.identifier.doi10.1073/pnas.73.11.4070
dc.identifier.pmid1069293
dc.identifier.urihttp://hdl.handle.net/20.500.14038/26540
dc.description.abstractTubulin purified by phosphocellulose chromatography and free of accessory proteins will not form microtubules in the absence or presence of microtubule initiating sites (flagellar microtubules). The capacity for growth onto pre-existing "seeds" can be restored by the addition of small quantities of partially purified tau protein. Larger quantities restore the capacity for spontaneous assembly. These results suggest that tubulin requires tau for both initiation and growth of microtubules and that tau is incorporated into the microtubule throughout its length.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=1069293&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC431332
dc.subjectAnimals
dc.subjectFlagella
dc.subjectGlycoproteins
dc.subjectKinetics
dc.subjectMicroscopy, Electron
dc.subjectMicrotubules
dc.subjectMolecular Weight
dc.subjectProteins
dc.subjectTubulin
dc.subjectAmino Acids, Peptides, and Proteins
dc.subjectCell Biology
dc.subjectInvestigative Techniques
dc.subjectMacromolecular Substances
dc.titleTubulin requires tau for growth onto microtubule initiating sites
dc.typeJournal Article
dc.source.journaltitleProceedings of the National Academy of Sciences of the United States of America
dc.source.volume73
dc.source.issue11
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/cellbiology_pp/44
dc.identifier.contextkey682197
html.description.abstract<p>Tubulin purified by phosphocellulose chromatography and free of accessory proteins will not form microtubules in the absence or presence of microtubule initiating sites (flagellar microtubules). The capacity for growth onto pre-existing "seeds" can be restored by the addition of small quantities of partially purified tau protein. Larger quantities restore the capacity for spontaneous assembly. These results suggest that tubulin requires tau for both initiation and growth of microtubules and that tau is incorporated into the microtubule throughout its length.</p>
dc.identifier.submissionpathcellbiology_pp/44
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages4070-4


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