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    Effects of adenylyl imidodiphosphate, a nonhydrolyzable adenosine triphosphate analog, on reactivated and rigor wave sea urchin sperm

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    Authors
    Penningroth, Stephen M.
    Witman, George B.
    UMass Chan Affiliations
    Department of Cell Biology
    Document Type
    Journal Article
    Publication Date
    1978-12-01
    Keywords
    Adenosine Triphosphate
    Adenylyl Imidodiphosphate
    Animals
    Dynein ATPase
    Male
    Microtubules
    Protein Binding
    Sea Urchins
    Sperm Motility
    Spermatozoa
    Animal Experimentation and Research
    Cell Biology
    Cells
    Heterocyclic Compounds
    Investigative Techniques
    Nucleic Acids, Nucleotides, and Nucleosides
    Urogenital System
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    Link to Full Text
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110271
    Abstract
    A nonhydrolyzable ATP analog, adenylyl imidodiphosphate (AMP-PNP), has been used to study the role of ATP binding in flagellar motility. Sea urchin sperm of Lytechinus pictus were demembranated, reactivated, and locked in "rigor waves" by a modification of the method of Gibbons and Gibbons (11). Rigor wave sperm relaxed within 2 min after addition of 4 micrometer ATP, and reactivated upon addition of 10-12 micrometer ATP. The beat frequency of the reactivated sperm varied with ATP concentration according to Michaelis-Menten kinetics ("Km" = 0.24 mM; "Vmax" = 44 Hz) and was competitively inhibited by AMP-PNP (Ki" approximately to 8.1 mM). Rigor wave sperm were completely relaxed (straightened) within 2 min by AMP-PNP at concentrations of 2-4 mM. The possibilities that relaxation in AMP-PNP was a result of ATP contamination, AMP-PNP hydrolysis, or lowering of the free Mg++ concentration were conclusively ruled out. The results suggest that dynein cross-bridge release is dependent upon ATP binding but not hydrolysis.
    Source

    J Cell Biol. 1978 Dec;79(3):827-32.

    DOI
    10.1083/jcb.79.3.827
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/26542
    PubMed ID
    153347
    Related Resources

    Link to Article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.1083/jcb.79.3.827
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