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dc.contributor.authorMoss, Anthony G.
dc.contributor.authorGatti, Jean-Luc
dc.contributor.authorKing, Stephen M.
dc.contributor.authorWitman, George B.
dc.date2022-08-11T08:08:04.000
dc.date.accessioned2022-08-23T15:41:09Z
dc.date.available2022-08-23T15:41:09Z
dc.date.issued1991-01-01
dc.date.submitted2008-12-15
dc.identifier.citation<p>Methods Enzymol. 1991;196:201-22.</p>
dc.identifier.issn0076-6879 (Print)
dc.identifier.doi10.1016/0076-6879(91)96020-R
dc.identifier.pmid1827864
dc.identifier.urihttp://hdl.handle.net/20.500.14038/26567
dc.description.abstractDyneins are multimeric ATPases, which make up the inner and outer arms that bridge the outer doublet microtubules of eukaryotic cilia and flagella. They are responsible for the generation of sliding between outer doublets, which in turn is the basis for the formation and propagation of bending waves in both cilia and flagella. Outer arm dyneins are composed of two to three ATPases of Mr > 400,000, referred to as the α, β, and, where appropriate, γ heavy chains. Trout sperm is a new source of vertebrate dynein. Sperm can be repeatedly obtained in large quantities from the same trout (up to 4 × 10 spermatozoa per ejaculate), their axonemes can be readily isolated, and the dynein can be extracted efficiently and without significant proteolytic degradation. The advantages of trout sperm have permitted the detailed characterization of trout outer arm dynein to progress rapidly, so that it is now one of the best characterized of all dyneins. This chapter presents an overview of trout physiology and spermatogenesis for those not well acquainted with teleost physiology and anatomy and describes the methods for purification and characterization of Salmo gairdneri outer arm dynein.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=1827864&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1016/0076-6879(91)96020-R
dc.subjectAnimals
dc.subjectCa(2+) Mg(2+)-ATPase
dc.subjectCell Fractionation
dc.subjectCentrifugation, Density Gradient
dc.subjectChromatography, Ion Exchange
dc.subjectDynein ATPase
dc.subjectElectrophoresis, Polyacrylamide Gel
dc.subjectIndicators and Reagents
dc.subjectKinetics
dc.subjectMacromolecular Substances
dc.subjectMale
dc.subjectMolecular Weight
dc.subjectSemen
dc.subjectSperm Motility
dc.subjectSperm Tail
dc.subjectSpermatogenesis
dc.subjectSpermatozoa
dc.subjectTestis
dc.subjectTrout
dc.subjectUltracentrifugation
dc.subjectAmino Acids, Peptides, and Proteins
dc.subjectCell Biology
dc.subjectCells
dc.subjectEnzymes and Coenzymes
dc.subjectUrogenital System
dc.titlePurification and characterization of Salmo gairdneri outer arm dynein
dc.typeBook Chapter
dc.source.booktitleMethods in enzymology
dc.source.volume196
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/cellbiology_pp/74
dc.identifier.contextkey682227
html.description.abstract<p>Dyneins are multimeric ATPases, which make up the inner and outer arms that bridge the outer doublet microtubules of eukaryotic cilia and flagella. They are responsible for the generation of sliding between outer doublets, which in turn is the basis for the formation and propagation of bending waves in both cilia and flagella. Outer arm dyneins are composed of two to three ATPases of M<sub>r</sub> > 400,000, referred to as the α, β, and, where appropriate, γ heavy chains. Trout sperm is a new source of vertebrate dynein. Sperm can be repeatedly obtained in large quantities from the same trout (up to 4 × 10 spermatozoa per ejaculate), their axonemes can be readily isolated, and the dynein can be extracted efficiently and without significant proteolytic degradation. The advantages of trout sperm have permitted the detailed characterization of trout outer arm dynein to progress rapidly, so that it is now one of the best characterized of all dyneins. This chapter presents an overview of trout physiology and spermatogenesis for those not well acquainted with teleost physiology and anatomy and describes the methods for purification and characterization of <em>Salmo gairdneri</em> outer arm dynein.</p>
dc.identifier.submissionpathcellbiology_pp/74
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages201-22


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