Ribonucleoprotein immunoprecipitation (RNP-IP): a direct in vivo analysis of microRNA-targets
Authors
Hassan, Mohammad Q.Gordon, Jonathan A. R.
Lian, Jane B.
Van Wijnen, Andre J.
Stein, Janet L.
Stein, Gary S.
UMass Chan Affiliations
Department of Cell Biology and Cancer CenterDocument Type
Journal ArticlePublication Date
2010-06-22
Metadata
Show full item recordAbstract
We have developed a novel Ribonucleoprotein Immunoprecipitation (RNP-IP) method to isolate miR-RISC complexes, associated microRNAs and target mRNAs. Our method characterizes mRNAs present in immunoprecipitates containing miR-RISC complexes that were obtained using GW182 and AGO2 antibodies. MicroRNA bound transcripts were reverse transcribed and amplified using seed sequence and 3'UTR derived primers. This flexible IP-based assay is a straightforward method to identify miRs participating in gene regulation and their cognate mRNAs in real time.Source
J Cell Biochem. 2010 Jul 1;110(4):817-22. Link to article on publisher's siteDOI
10.1002/jcb.22562Permanent Link to this Item
http://hdl.handle.net/20.500.14038/26585PubMed ID
20564179Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1002/jcb.22562