Ribonucleoprotein immunoprecipitation (RNP-IP): a direct in vivo analysis of microRNA-targets
AuthorsHassan, Mohammad Q.
Gordon, Jonathan A. R.
Lian, Jane B.
Van Wijnen, Andre J.
Stein, Janet L.
Stein, Gary S.
UMass Chan AffiliationsDepartment of Cell Biology and Cancer Center
Document TypeJournal Article
MetadataShow full item record
AbstractWe have developed a novel Ribonucleoprotein Immunoprecipitation (RNP-IP) method to isolate miR-RISC complexes, associated microRNAs and target mRNAs. Our method characterizes mRNAs present in immunoprecipitates containing miR-RISC complexes that were obtained using GW182 and AGO2 antibodies. MicroRNA bound transcripts were reverse transcribed and amplified using seed sequence and 3'UTR derived primers. This flexible IP-based assay is a straightforward method to identify miRs participating in gene regulation and their cognate mRNAs in real time.
SourceJ Cell Biochem. 2010 Jul 1;110(4):817-22. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/26585
Related ResourcesLink to Article in PubMed