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dc.contributor.authorHassan, Mohammad Q.
dc.contributor.authorGordon, Jonathan A. R.
dc.contributor.authorLian, Jane B.
dc.contributor.authorVan Wijnen, Andre J.
dc.contributor.authorStein, Janet L.
dc.contributor.authorStein, Gary S.
dc.date2022-08-11T08:08:04.000
dc.date.accessioned2022-08-23T15:41:14Z
dc.date.available2022-08-23T15:41:14Z
dc.date.issued2010-06-22
dc.date.submitted2010-08-03
dc.identifier.citationJ Cell Biochem. 2010 Jul 1;110(4):817-22. <a href="http://dx.doi.org/10.1002/jcb.22562">Link to article on publisher's site</a>
dc.identifier.issn0730-2312 (Linking)
dc.identifier.doi10.1002/jcb.22562
dc.identifier.pmid20564179
dc.identifier.urihttp://hdl.handle.net/20.500.14038/26585
dc.description.abstractWe have developed a novel Ribonucleoprotein Immunoprecipitation (RNP-IP) method to isolate miR-RISC complexes, associated microRNAs and target mRNAs. Our method characterizes mRNAs present in immunoprecipitates containing miR-RISC complexes that were obtained using GW182 and AGO2 antibodies. MicroRNA bound transcripts were reverse transcribed and amplified using seed sequence and 3'UTR derived primers. This flexible IP-based assay is a straightforward method to identify miRs participating in gene regulation and their cognate mRNAs in real time.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=20564179&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1002/jcb.22562
dc.subjectImmunoprecipitation
dc.subjectRibonucleoproteins
dc.subjectMicroRNAs
dc.subjectCell Biology
dc.titleRibonucleoprotein immunoprecipitation (RNP-IP): a direct in vivo analysis of microRNA-targets
dc.typeJournal Article
dc.source.journaltitleJournal of cellular biochemistry
dc.source.volume110
dc.source.issue4
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/cellbiology_pp/92
dc.identifier.contextkey1421243
html.description.abstract<p>We have developed a novel Ribonucleoprotein Immunoprecipitation (RNP-IP) method to isolate miR-RISC complexes, associated microRNAs and target mRNAs. Our method characterizes mRNAs present in immunoprecipitates containing miR-RISC complexes that were obtained using GW182 and AGO2 antibodies. MicroRNA bound transcripts were reverse transcribed and amplified using seed sequence and 3'UTR derived primers. This flexible IP-based assay is a straightforward method to identify miRs participating in gene regulation and their cognate mRNAs in real time.</p>
dc.identifier.submissionpathcellbiology_pp/92
dc.contributor.departmentDepartment of Cell Biology and Cancer Center
dc.source.pages817-22


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