Ribonucleoprotein immunoprecipitation (RNP-IP): a direct in vivo analysis of microRNA-targets
dc.contributor.author | Hassan, Mohammad Q. | |
dc.contributor.author | Gordon, Jonathan A. R. | |
dc.contributor.author | Lian, Jane B. | |
dc.contributor.author | Van Wijnen, Andre J. | |
dc.contributor.author | Stein, Janet L. | |
dc.contributor.author | Stein, Gary S. | |
dc.date | 2022-08-11T08:08:04.000 | |
dc.date.accessioned | 2022-08-23T15:41:14Z | |
dc.date.available | 2022-08-23T15:41:14Z | |
dc.date.issued | 2010-06-22 | |
dc.date.submitted | 2010-08-03 | |
dc.identifier.citation | J Cell Biochem. 2010 Jul 1;110(4):817-22. <a href="http://dx.doi.org/10.1002/jcb.22562">Link to article on publisher's site</a> | |
dc.identifier.issn | 0730-2312 (Linking) | |
dc.identifier.doi | 10.1002/jcb.22562 | |
dc.identifier.pmid | 20564179 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/26585 | |
dc.description.abstract | We have developed a novel Ribonucleoprotein Immunoprecipitation (RNP-IP) method to isolate miR-RISC complexes, associated microRNAs and target mRNAs. Our method characterizes mRNAs present in immunoprecipitates containing miR-RISC complexes that were obtained using GW182 and AGO2 antibodies. MicroRNA bound transcripts were reverse transcribed and amplified using seed sequence and 3'UTR derived primers. This flexible IP-based assay is a straightforward method to identify miRs participating in gene regulation and their cognate mRNAs in real time. | |
dc.language.iso | en_US | |
dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=20564179&dopt=Abstract">Link to Article in PubMed</a> | |
dc.relation.url | http://dx.doi.org/10.1002/jcb.22562 | |
dc.subject | Immunoprecipitation | |
dc.subject | Ribonucleoproteins | |
dc.subject | MicroRNAs | |
dc.subject | Cell Biology | |
dc.title | Ribonucleoprotein immunoprecipitation (RNP-IP): a direct in vivo analysis of microRNA-targets | |
dc.type | Journal Article | |
dc.source.journaltitle | Journal of cellular biochemistry | |
dc.source.volume | 110 | |
dc.source.issue | 4 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/cellbiology_pp/92 | |
dc.identifier.contextkey | 1421243 | |
html.description.abstract | <p>We have developed a novel Ribonucleoprotein Immunoprecipitation (RNP-IP) method to isolate miR-RISC complexes, associated microRNAs and target mRNAs. Our method characterizes mRNAs present in immunoprecipitates containing miR-RISC complexes that were obtained using GW182 and AGO2 antibodies. MicroRNA bound transcripts were reverse transcribed and amplified using seed sequence and 3'UTR derived primers. This flexible IP-based assay is a straightforward method to identify miRs participating in gene regulation and their cognate mRNAs in real time.</p> | |
dc.identifier.submissionpath | cellbiology_pp/92 | |
dc.contributor.department | Department of Cell Biology and Cancer Center | |
dc.source.pages | 817-22 |