Identification of T Cell Epitopes in the Spike Glycoprotein of Severe Acute Respiratory Syndrome Coronavirus 2 in Rhesus Macaques
Hu, Zhengrong R.
He, Zhanlong L.
UMass Chan AffiliationsDepartment of Medicine
Document TypeJournal Article
KeywordsSevere Acute Respiratory Syndrome Coronavirus 2
T cell epitopes
Amino Acids, Peptides, and Proteins
Immunology of Infectious Disease
Immunoprophylaxis and Therapy
MetadataShow full item record
AbstractThe T cell response is an important detection index in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine development. The present study was undertaken to determine the T cell epitopes in the spike (S) protein of SARS-CoV-2 that dominate the T cell responses in SARS-CoV-2-infected patients. PBMCs from rhesus macaques vaccinated with a DNA vaccine encoding the full-length S protein were isolated, and an ELISPOT assay was used to identify the recognized T cell epitopes among a total of 158 18-mer and 10-aa-overlapping peptides spanning the full-length S protein. Six multipeptide-based epitopes located in the S1 region, with four of the six located in the receptor-binding domain, were defined as the most frequently recognized epitopes in macaques. The conservation of the epitopes across species was also verified, and peptide mixtures for T cell response detection were established. Six newly defined T cell epitopes were found in the current study, which may provide a novel potential target for T cell response detection and the diagnosis and vaccine design of SARS-CoV-2 based on multipeptide subunit-based epitopes.
Liu X, Li Y, Xiao H, Bi Y, Gong Y, R Hu Z, Zeng Y, Sun M, L He Z, Lu S, Li Q, Cun W. Identification of T Cell Epitopes in the Spike Glycoprotein of Severe Acute Respiratory Syndrome Coronavirus 2 in Rhesus Macaques. J Immunol. 2021 May 12:ji2000922. doi: 10.4049/jimmunol.2000922. Epub ahead of print. PMID: 33980582. Link to article on publisher's site