Identification of T Cell Epitopes in the Spike Glycoprotein of Severe Acute Respiratory Syndrome Coronavirus 2 in Rhesus Macaques
Authors
Liu, XiaojuanLi, Yuzhong
Xiao, Hongjian
Bi, Yanwei
Gong, Yue
Hu, Zhengrong R.
Zeng, Yaxin
Sun, Ming
He, Zhanlong L.
Lu, Shan
Li, Qihan
Cun, Wei
UMass Chan Affiliations
Department of MedicineDocument Type
Journal ArticlePublication Date
2021-05-12Keywords
Severe Acute Respiratory Syndrome Coronavirus 2SARS-CoV-2
vaccine development
T cell epitopes
spike glycoprotein
Amino Acids, Peptides, and Proteins
Immunology of Infectious Disease
Immunopathology
Immunoprophylaxis and Therapy
Immunotherapy
Infectious Disease
Microbiology
Virus Diseases
Metadata
Show full item recordAbstract
The T cell response is an important detection index in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine development. The present study was undertaken to determine the T cell epitopes in the spike (S) protein of SARS-CoV-2 that dominate the T cell responses in SARS-CoV-2-infected patients. PBMCs from rhesus macaques vaccinated with a DNA vaccine encoding the full-length S protein were isolated, and an ELISPOT assay was used to identify the recognized T cell epitopes among a total of 158 18-mer and 10-aa-overlapping peptides spanning the full-length S protein. Six multipeptide-based epitopes located in the S1 region, with four of the six located in the receptor-binding domain, were defined as the most frequently recognized epitopes in macaques. The conservation of the epitopes across species was also verified, and peptide mixtures for T cell response detection were established. Six newly defined T cell epitopes were found in the current study, which may provide a novel potential target for T cell response detection and the diagnosis and vaccine design of SARS-CoV-2 based on multipeptide subunit-based epitopes.Source
Liu X, Li Y, Xiao H, Bi Y, Gong Y, R Hu Z, Zeng Y, Sun M, L He Z, Lu S, Li Q, Cun W. Identification of T Cell Epitopes in the Spike Glycoprotein of Severe Acute Respiratory Syndrome Coronavirus 2 in Rhesus Macaques. J Immunol. 2021 May 12:ji2000922. doi: 10.4049/jimmunol.2000922. Epub ahead of print. PMID: 33980582. Link to article on publisher's site
DOI
10.4049/jimmunol.2000922Permanent Link to this Item
http://hdl.handle.net/20.500.14038/27434PubMed ID
33980582Related Resources
ae974a485f413a2113503eed53cd6c53
10.4049/jimmunol.2000922