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    Identification of T Cell Epitopes in the Spike Glycoprotein of Severe Acute Respiratory Syndrome Coronavirus 2 in Rhesus Macaques

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    Authors
    Liu, Xiaojuan
    Li, Yuzhong
    Xiao, Hongjian
    Bi, Yanwei
    Gong, Yue
    Hu, Zhengrong R.
    Zeng, Yaxin
    Sun, Ming
    He, Zhanlong L.
    Lu, Shan
    Li, Qihan
    Cun, Wei
    Show allShow less
    UMass Chan Affiliations
    Department of Medicine
    Document Type
    Journal Article
    Publication Date
    2021-05-12
    Keywords
    Severe Acute Respiratory Syndrome Coronavirus 2
    SARS-CoV-2
    vaccine development
    T cell epitopes
    spike glycoprotein
    Amino Acids, Peptides, and Proteins
    Immunology of Infectious Disease
    Immunopathology
    Immunoprophylaxis and Therapy
    Immunotherapy
    Infectious Disease
    Microbiology
    Virus Diseases
    Show allShow less
    
    Metadata
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    Link to Full Text
    https://doi.org/10.4049/jimmunol.2000922
    Abstract
    The T cell response is an important detection index in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine development. The present study was undertaken to determine the T cell epitopes in the spike (S) protein of SARS-CoV-2 that dominate the T cell responses in SARS-CoV-2-infected patients. PBMCs from rhesus macaques vaccinated with a DNA vaccine encoding the full-length S protein were isolated, and an ELISPOT assay was used to identify the recognized T cell epitopes among a total of 158 18-mer and 10-aa-overlapping peptides spanning the full-length S protein. Six multipeptide-based epitopes located in the S1 region, with four of the six located in the receptor-binding domain, were defined as the most frequently recognized epitopes in macaques. The conservation of the epitopes across species was also verified, and peptide mixtures for T cell response detection were established. Six newly defined T cell epitopes were found in the current study, which may provide a novel potential target for T cell response detection and the diagnosis and vaccine design of SARS-CoV-2 based on multipeptide subunit-based epitopes.
    Source

    Liu X, Li Y, Xiao H, Bi Y, Gong Y, R Hu Z, Zeng Y, Sun M, L He Z, Lu S, Li Q, Cun W. Identification of T Cell Epitopes in the Spike Glycoprotein of Severe Acute Respiratory Syndrome Coronavirus 2 in Rhesus Macaques. J Immunol. 2021 May 12:ji2000922. doi: 10.4049/jimmunol.2000922. Epub ahead of print. PMID: 33980582. Link to article on publisher's site

    DOI
    10.4049/jimmunol.2000922
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/27434
    PubMed ID
    33980582
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    Link to Article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.4049/jimmunol.2000922
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