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dc.contributor.authorNarayanaswamy, Vignesh
dc.contributor.authorPentecost, Brian
dc.contributor.authorAlfandari, Dominique
dc.contributor.authorChin, Emily
dc.contributor.authorMinor, Kathleen
dc.contributor.authorKastrinakis, Alyssa
dc.contributor.authorLieberman, Tanya
dc.contributor.authorArcaro, Kathleen F.
dc.contributor.authorLeftwich, Heidi K
dc.date2022-08-11T08:08:10.000
dc.date.accessioned2022-08-23T15:45:15Z
dc.date.available2022-08-23T15:45:15Z
dc.date.issued2021-08-11
dc.date.submitted2021-08-27
dc.identifier.citation<p>Narayanaswamy V, Pentecost B, Alfandari D, Chin E, Minor K, Kastrinakis A, Lieberman T, Arcaro KF, Leftwich H. Humoral and Cell-Mediated Immune Response in Colostrum from Women Diagnosed Positive for SARS-CoV-2. Breastfeed Med. 2021 Aug 11. doi: 10.1089/bfm.2021.0082. Epub ahead of print. PMID: 34382820. <a href="https://doi.org/10.1089/bfm.2021.0082">Link to article on publisher's site</a></p>
dc.identifier.issn1556-8253 (Linking)
dc.identifier.doi10.1089/bfm.2021.0082
dc.identifier.pmid34382820
dc.identifier.urihttp://hdl.handle.net/20.500.14038/27486
dc.description.abstractObjective: To evaluate the immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in colostrum from women who tested positive for the virus. Methods: Between March and September 2020 we obtained bilateral colostrum samples collected on spot cards within 48 hours of delivery from 15 new mothers who had previously tested positive for SARS-CoV-2. Four of 15 women provided liquid colostrum, which was used for validating results obtained from spot cards. Archived bilateral colostrum samples collected from 8 women during 2011-2013 were used as pre-coronavirus disease 2019 (COVID-19) controls. All samples were tested for reactivity to the receptor binding domain (RBD) of the SARS-CoV-2 spike protein using an enzyme-linked immunosorbent assay that measures SARS-CoV-2 RBD-specific IgA, IgG, and IgM and for levels of 10 inflammatory cytokines (interferon-gamma [IFN-gamma], tumor necrosis factor-alpha, interleukin [IL]-1beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-13) using a multiplex electrochemiluminescent sandwich assay. Results: Our validation studies indicate that the levels of SARS-CoV-2-specific antibodies and the associated cytokines measured in liquid colostrum are comparable to levels eluted from spot cards. Bilateral colostrum samples from 73%, 73%, and 33% of the 15 COVID-19 mothers exhibited IgA, IgG, and IgM reactivity to RBD, respectively. In addition, symptomatic COVID-19 mothers had statistically significant elevated levels of 4 of the 10 inflammatory markers (IFN-gamma, IL-4, IL-6, and IL-12) compared to asymptomatic COVID-19 mothers. Conclusions: A strong humoral immune response is present in the colostrum of women who were infected with SARS-CoV-2 before delivering. The evolution and duration of the antibody response, as well as dynamics of the cytokine response, remain to be determined. Our results also indicate that future large-scale studies can be conducted with milk easily collected on paper spot cards.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=34382820&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright Vignesh Narayanaswamy et al. 2021; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons License [CC-BY] (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCOVID-19
dc.subjectSARS-CoV-2
dc.subjectantibody
dc.subjectbreast milk
dc.subjectbreastfeeding
dc.subjectcolostrum
dc.subjectcytokine
dc.subjectHemic and Immune Systems
dc.subjectImmunology of Infectious Disease
dc.subjectInfectious Disease
dc.subjectMaternal and Child Health
dc.subjectMicrobiology
dc.subjectVirus Diseases
dc.titleHumoral and Cell-Mediated Immune Response in Colostrum from Women Diagnosed Positive for SARS-CoV-2
dc.typeJournal Article
dc.source.journaltitleBreastfeeding medicine : the official journal of the Academy of Breastfeeding Medicine
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1294&amp;context=covid19&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/covid19/289
dc.identifier.contextkey24527831
refterms.dateFOA2022-08-23T15:45:15Z
html.description.abstract<p>Objective: To evaluate the immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in colostrum from women who tested positive for the virus.</p> <p>Methods: Between March and September 2020 we obtained bilateral colostrum samples collected on spot cards within 48 hours of delivery from 15 new mothers who had previously tested positive for SARS-CoV-2. Four of 15 women provided liquid colostrum, which was used for validating results obtained from spot cards. Archived bilateral colostrum samples collected from 8 women during 2011-2013 were used as pre-coronavirus disease 2019 (COVID-19) controls. All samples were tested for reactivity to the receptor binding domain (RBD) of the SARS-CoV-2 spike protein using an enzyme-linked immunosorbent assay that measures SARS-CoV-2 RBD-specific IgA, IgG, and IgM and for levels of 10 inflammatory cytokines (interferon-gamma [IFN-gamma], tumor necrosis factor-alpha, interleukin [IL]-1beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-13) using a multiplex electrochemiluminescent sandwich assay.</p> <p>Results: Our validation studies indicate that the levels of SARS-CoV-2-specific antibodies and the associated cytokines measured in liquid colostrum are comparable to levels eluted from spot cards. Bilateral colostrum samples from 73%, 73%, and 33% of the 15 COVID-19 mothers exhibited IgA, IgG, and IgM reactivity to RBD, respectively. In addition, symptomatic COVID-19 mothers had statistically significant elevated levels of 4 of the 10 inflammatory markers (IFN-gamma, IL-4, IL-6, and IL-12) compared to asymptomatic COVID-19 mothers.</p> <p>Conclusions: A strong humoral immune response is present in the colostrum of women who were infected with SARS-CoV-2 before delivering. The evolution and duration of the antibody response, as well as dynamics of the cytokine response, remain to be determined. Our results also indicate that future large-scale studies can be conducted with milk easily collected on paper spot cards.</p>
dc.identifier.submissionpathcovid19/289
dc.contributor.departmentSchool of Medicine
dc.contributor.departmentDepartment of Obstetrics and Gynecology, Division of Maternal-Fetal Medicine


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Copyright Vignesh Narayanaswamy et al. 2021; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons License [CC-BY] (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as Copyright Vignesh Narayanaswamy et al. 2021; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons License [CC-BY] (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.