Isolation, electron microscopy and 3D reconstruction of invertebrate muscle myofilaments
Name:
Publisher version
View Source
Access full-text PDFOpen Access
View Source
Check access options
Check access options
Authors
Craig, Roger W.UMass Chan Affiliations
Department of Cell BiologyDocument Type
Journal ArticlePublication Date
2012-01-14Keywords
Muscle ContractionMyofibrils
Invertebrates
Microscopy, Electron
Image Processing, Computer-Assisted
Cell Biology
Metadata
Show full item recordAbstract
Understanding the molecular mechanism of muscle contraction and its regulation has been greatly influenced and aided by studies of myofilament structure in invertebrate muscles. Invertebrates are easily obtained and cover a broad spectrum of species and functional specializations. The thick (myosin-containing) filaments from some invertebrates are especially stable and simple in structure and thus much more amenable to structural analysis than those of vertebrates. Comparative studies of invertebrate filaments by electron microscopy and image processing have provided important generalizations of muscle molecular structure and function. This article reviews methods for preparing thick and thin filaments from invertebrate muscle, for imaging filaments by electron microscopy, and for determining their three dimensional structure by image processing. It also highlights some of the key insights into filament function that have come from these studies.Source
Methods. 2012 Jan;56(1):33-43. Epub 2011 Dec 2. Link to article on publisher's siteDOI
10.1016/j.ymeth.2011.11.007Permanent Link to this Item
http://hdl.handle.net/20.500.14038/27668PubMed ID
22155190Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1016/j.ymeth.2011.11.007