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dc.contributor.authorGalinska-Rakoczy, Agnieszka
dc.contributor.authorEngel, Patti
dc.contributor.authorXu, Chen
dc.contributor.authorJung, HyunSuk
dc.contributor.authorCraig, Roger W.
dc.contributor.authorTobacman, Larry S.
dc.contributor.authorLehman, William
dc.date2022-08-11T08:08:12.000
dc.date.accessioned2022-08-23T15:46:06Z
dc.date.available2022-08-23T15:46:06Z
dc.date.issued2008-06-03
dc.date.submitted2010-10-06
dc.identifier.citationJ Mol Biol. 2008 Jun 20;379(5):929-35. Epub 2008 May 3. <a href="http://dx.doi.org/10.1016/j.jmb.2008.04.062">Link to article on publisher's site</a>
dc.identifier.issn0022-2836 (Linking)
dc.identifier.doi10.1016/j.jmb.2008.04.062
dc.identifier.pmid18514658
dc.identifier.urihttp://hdl.handle.net/20.500.14038/27684
dc.description.abstractThe molecular switching mechanism governing skeletal and cardiac muscle contraction couples the binding of Ca2+ on troponin to the movement of tropomyosin on actin filaments. Despite years of investigation, this mechanism remains unclear because it has not yet been possible to directly assess the structural influence of troponin on tropomyosin that causes actin filaments, and hence myosin-crossbridge cycling and contraction, to switch on and off. A C-terminal domain of troponin I is thought to be intimately involved in inducing tropomyosin movement to an inhibitory position that blocks myosin-crossbridge interaction. Release of this regulatory, latching domain from actin after Ca2+ binding to TnC (the Ca2+ sensor of troponin that relieves inhibition) presumably allows tropomyosin movement away from the inhibitory position on actin, thus initiating contraction. However, the structural interactions of the regulatory domain of TnI (the "inhibitory" subunit of troponin) with tropomyosin and actin that cause tropomyosin movement are unknown, and thus, the regulatory process is not well defined. Here, thin filaments were labeled with an engineered construct representing C-terminal TnI, and then, 3D electron microscopy was used to resolve where troponin is anchored on actin-tropomyosin. Electron microscopy reconstruction showed how TnI binding to both actin and tropomyosin at low Ca2+ competes with tropomyosin for a common site on actin and drives tropomyosin movement to a constrained, relaxing position to inhibit myosin-crossbridge association. Thus, the observations reported reveal the structural mechanism responsible for troponin-tropomyosin-mediated steric interference of actin-myosin interaction that regulates muscle contraction.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=18514658&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1016/j.jmb.2008.04.062
dc.subjectActins
dc.subjectHumans
dc.subjectImage Processing, Computer-Assisted
dc.subjectMicroscopy, Electron, Transmission
dc.subjectModels, Biological
dc.subjectModels, Molecular
dc.subjectMultiprotein Complexes
dc.subjectMuscle Contraction
dc.subjectProtein Engineering
dc.subjectProtein Structure, Quaternary
dc.subjectRecombinant Proteins
dc.subjectTropomyosin
dc.subjectTroponin
dc.subjectTroponin I
dc.subjectCell Biology
dc.titleStructural basis for the regulation of muscle contraction by troponin and tropomyosin
dc.typeArticle
dc.source.journaltitleJournal of molecular biology
dc.source.volume379
dc.source.issue5
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/craig/9
dc.identifier.contextkey1594908
html.description.abstract<p>The molecular switching mechanism governing skeletal and cardiac muscle contraction couples the binding of Ca2+ on troponin to the movement of tropomyosin on actin filaments. Despite years of investigation, this mechanism remains unclear because it has not yet been possible to directly assess the structural influence of troponin on tropomyosin that causes actin filaments, and hence myosin-crossbridge cycling and contraction, to switch on and off. A C-terminal domain of troponin I is thought to be intimately involved in inducing tropomyosin movement to an inhibitory position that blocks myosin-crossbridge interaction. Release of this regulatory, latching domain from actin after Ca2+ binding to TnC (the Ca2+ sensor of troponin that relieves inhibition) presumably allows tropomyosin movement away from the inhibitory position on actin, thus initiating contraction. However, the structural interactions of the regulatory domain of TnI (the "inhibitory" subunit of troponin) with tropomyosin and actin that cause tropomyosin movement are unknown, and thus, the regulatory process is not well defined. Here, thin filaments were labeled with an engineered construct representing C-terminal TnI, and then, 3D electron microscopy was used to resolve where troponin is anchored on actin-tropomyosin. Electron microscopy reconstruction showed how TnI binding to both actin and tropomyosin at low Ca2+ competes with tropomyosin for a common site on actin and drives tropomyosin movement to a constrained, relaxing position to inhibit myosin-crossbridge association. Thus, the observations reported reveal the structural mechanism responsible for troponin-tropomyosin-mediated steric interference of actin-myosin interaction that regulates muscle contraction.</p>
dc.identifier.submissionpathcraig/9
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages929-35


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