Targeted Mutagenesis of a Therapeutic Human Monoclonal IgG1 Antibody Prevents Gelation at High Concentrations
Boucher, Elisabeth N.
Ozturk, Sadettin S.
Thomas, William D. Jr.
Document TypePoster Abstract
KeywordsAmino Acids, Peptides, and Proteins
Immunoprophylaxis and Therapy
Translational Medical Research
MetadataShow full item record
AbstractA common challenge encountered during development of high concentration monoclonal antibody formulations is preventing self-association. Depending on the antibody and its formulation, self-association can be seen as aggregation, precipitation, opalescence or phase separation. Here we report on an unusual manifestation of self-association, formation of a semi-solid gel or “gelation”. Therapeutic monoclonal antibody C4 was isolated from human B cells based on its strong potency in neutralizing bacterial toxin in animal models. The purified antibody possessed the unusual property of forming a firm, opaque white gel when it was formulated at concentrations >40 mg/mL and the temperature was
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/27940
Abstract of poster presented at the 2014 UMass Center for Clinical and Translational Science Research Retreat, held on May 20, 2014 at the University of Massachusetts Medical School, Worcester, Mass.
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