GW182 controls Drosophila circadian behavior and PDF-receptor signaling
Name:
Publisher version
View Source
Access full-text PDFOpen Access
View Source
Check access options
Check access options
Document Type
Journal ArticlePublication Date
2013-04-10Keywords
AnimalsAnimals, Genetically Modified
Circadian Rhythm
Drosophila
Drosophila Proteins
Gene Expression Regulation, Developmental
Green Fluorescent Proteins
Locomotion
Male
MicroRNAs
RNA Interference
Receptors, G-Protein-Coupled
Signal Transduction
Temperature
Transcription Factors
Molecular and Cellular Neuroscience
Neuroscience and Neurobiology
Metadata
Show full item recordAbstract
The neuropeptide PDF is crucial for Drosophila circadian behavior: it keeps circadian neurons synchronized. Here, we identify GW182 as a key regulator of PDF signaling. Indeed, GW182 downregulation results in phenotypes similar to those of Pdf and Pdf-receptor (Pdfr) mutants. gw182 genetically interacts with Pdfr and cAMP signaling, which is essential for PDFR function. GW182 mediates miRNA-dependent gene silencing through its interaction with AGO1. Consistently, GW182's AGO1 interaction domain is required for GW182's circadian function. Moreover, our results indicate that GW182 modulates PDFR signaling by silencing the expression of the cAMP phosphodiesterase DUNCE. Importantly, this repression is under photic control, and GW182 activity level--which is limiting in circadian neurons--influences the responses of the circadian neural network to light. We propose that GW182's gene silencing activity functions as a rheostat for PDFR signaling and thus profoundly impacts the circadian neural network and its response to environmental inputs.Source
Neuron. 2013 Apr 10;78(1):152-65. doi: 10.1016/j.neuron.2013.01.035. Link to article on publisher's siteDOI
10.1016/j.neuron.2013.01.035Permanent Link to this Item
http://hdl.handle.net/20.500.14038/28758PubMed ID
23583112Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1016/j.neuron.2013.01.035