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dc.contributor.authorGammon, Don B.
dc.contributor.authorIshidate, Takao
dc.contributor.authorLi, Lichao
dc.contributor.authorGu, Weifeng
dc.contributor.authorSilverman, Neal S.
dc.contributor.authorMello, Craig C.
dc.date2022-08-11T08:08:21.000
dc.date.accessioned2022-08-23T15:52:06Z
dc.date.available2022-08-23T15:52:06Z
dc.date.issued2017-03-20
dc.date.submitted2017-06-02
dc.identifier.citation<p>Curr Biol. 2017 Mar 20;27(6):795-806. doi: 10.1016/j.cub.2017.02.004. Epub 2017 Mar 2. <a href="https://doi.org/10.1016/j.cub.2017.02.004">Link to article on publisher's site</a></p>
dc.identifier.issn0960-9822 (Linking)
dc.identifier.doi10.1016/j.cub.2017.02.004
dc.identifier.pmid28262484
dc.identifier.urihttp://hdl.handle.net/20.500.14038/29064
dc.description.abstractThe recent discovery of the positive-sense single-stranded RNA (ssRNA) Orsay virus (OV) as a natural pathogen of the nematode Caenorhabditis elegans has stimulated interest in exploring virus-nematode interactions. However, OV infection is restricted to a small number of intestinal cells, even in nematodes defective in their antiviral RNA interference (RNAi) response, and is neither lethal nor vertically transmitted. Using a fluorescent reporter strain of the negative-sense ssRNA vesicular stomatitis virus (VSV), we show that microinjection of VSV particles leads to a dose-dependent, muscle tissue-tropic, lethal infection in C. elegans. Furthermore, we find nematodes deficient for components of the antiviral RNAi pathway, such as Dicer-related helicase 1 (DRH-1), to display hypersusceptibility to VSV infection as evidenced by elevated infection rates, virus replication in multiple tissue types, and earlier mortality. Strikingly, infection of oocytes and embryos could also be observed in drh-1 mutants. Our results suggest that the antiviral RNAi response not only inhibits vertical VSV transmission but also promotes transgenerational inheritance of antiviral immunity. Our study introduces a new, in vivo virus-host model system for exploring arbovirus pathogenesis and provides the first evidence for vertical pathogen transmission in C. elegans.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=28262484&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright 2017 The Authors. Published by Elsevier Ltd.
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCaenorhabditis elegans
dc.subjectRNA interference
dc.subjectantiviral immunity
dc.subjectarbovirus
dc.subjectsmall RNAs
dc.subjecttransgenerational inheritance
dc.subjectvertical transmission
dc.subjectvesicular stomatitis virus
dc.subjectvirus-host interactions
dc.subjectAnimal Experimentation and Research
dc.subjectImmunity
dc.subjectImmunology of Infectious Disease
dc.subjectImmunopathology
dc.subjectImmunoprophylaxis and Therapy
dc.subjectMicrobiology
dc.subjectNucleic Acids, Nucleotides, and Nucleosides
dc.subjectVirology
dc.subjectViruses
dc.titleThe Antiviral RNA Interference Response Provides Resistance to Lethal Arbovirus Infection and Vertical Transmission in Caenorhabditis elegans
dc.typeJournal Article
dc.source.journaltitleCurrent biology : CB
dc.source.volume27
dc.source.issue6
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2295&amp;context=faculty_pubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/faculty_pubs/1292
dc.identifier.contextkey10243081
refterms.dateFOA2022-08-23T15:52:06Z
html.description.abstract<p>The recent discovery of the positive-sense single-stranded RNA (ssRNA) Orsay virus (OV) as a natural pathogen of the nematode Caenorhabditis elegans has stimulated interest in exploring virus-nematode interactions. However, OV infection is restricted to a small number of intestinal cells, even in nematodes defective in their antiviral RNA interference (RNAi) response, and is neither lethal nor vertically transmitted. Using a fluorescent reporter strain of the negative-sense ssRNA vesicular stomatitis virus (VSV), we show that microinjection of VSV particles leads to a dose-dependent, muscle tissue-tropic, lethal infection in C. elegans. Furthermore, we find nematodes deficient for components of the antiviral RNAi pathway, such as Dicer-related helicase 1 (DRH-1), to display hypersusceptibility to VSV infection as evidenced by elevated infection rates, virus replication in multiple tissue types, and earlier mortality. Strikingly, infection of oocytes and embryos could also be observed in drh-1 mutants. Our results suggest that the antiviral RNAi response not only inhibits vertical VSV transmission but also promotes transgenerational inheritance of antiviral immunity. Our study introduces a new, in vivo virus-host model system for exploring arbovirus pathogenesis and provides the first evidence for vertical pathogen transmission in C. elegans.</p>
dc.identifier.submissionpathfaculty_pubs/1292
dc.contributor.departmentDepartment of Medicine, Division of Infectious Diseases and Immunology
dc.contributor.departmentRNA Therapeutics Institute
dc.source.pages795-806


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Copyright 2017 The Authors. Published by Elsevier Ltd.
Except where otherwise noted, this item's license is described as Copyright 2017 The Authors. Published by Elsevier Ltd.