Simultaneous optogenetic manipulation and calcium imaging in freely moving C. elegans [preprint]

Shipley, Frederick B.; Clark, Christopher M.; Alkema, Mark J; Leifer, Andrew M.

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Authors

Shipley, Frederick B.
Clark, Christopher M.
Alkema, Mark J
Leifer, Andrew M.

Student Authors

Christopher M. Clark

Academic Program

Neuroscience

UMass Chan Affiliations

Morningside Graduate School of Biomedical Sciences
Alkema Lab
Neurobiology

Document Type

Preprint

Publication Date

2014-04-02

Keywords

optogenetics
systems neuroscience
calcium imaging
C. elegans
Channelrhodopsin
GCaMP3
neuroscience
Neuroscience and Neurobiology
Systems Neuroscience

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Abstract

A fundamental goal of systems neuroscience is to probe the dynamics of neural activity that drive behavior. Here we present an instrument to simultaneously manipulate neural activity via Channelrhodopsin, monitor neural response via GCaMP3, and observes behavior in freely moving C. elegans. We use the instrument to directly observe the relation between sensory stimuli, interneuron activity and locomotion in the mechanosensory circuit. Now published as: Front Neural Circuits 8:28, doi:10.3389/fncir.2014.00028

Source

bioRxiv 000943; doi: https://doi.org/10.1101/000943. Link to preprint on bioRxiv service.

DOI

10.1101/000943

Permanent Link to this Item

http://hdl.handle.net/20.500.14038/29286

Related Resources

Now published in Front Neural Circuits 8:28, doi:10.3389/fncir.2014.00028

Rights

The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC 4.0 International license.

Distribution License

http://creativecommons.org/licenses/by-nc/4.0/

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Morningside GSBS Scholarly Publications

Neurobiology Student Publications

UMass Chan Faculty and Researcher Publications

Neurobiology Faculty Publications

The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC 4.0 International license.

Except where otherwise noted, this item's license is described as The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC 4.0 International license.