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dc.contributor.authorBoag, Peter R.
dc.contributor.authorDavis, Gregory M.
dc.contributor.authorTu, Shikui
dc.contributor.authorColson, Rhys N.
dc.contributor.authorAnderson, Joshua W. T.
dc.contributor.authorGunzburg, Menachem J.
dc.contributor.authorFrancisco, Michelle A.
dc.contributor.authorRay, Debashish
dc.contributor.authorMaity, Tuhin
dc.contributor.authorWu, Monica Z.
dc.contributor.authorMorris, Quaid D.
dc.contributor.authorHughes, Timothy R.
dc.contributor.authorWilce, Jacqueline A.
dc.contributor.authorUniversity of Toronto
dc.contributor.authorWeng, Zhiping
dc.date2022-08-11T08:08:23.000
dc.date.accessioned2022-08-23T15:53:15Z
dc.date.available2022-08-23T15:53:15Z
dc.date.issued2018-02-05
dc.date.submitted2018-06-14
dc.identifier.citation<p>bioRxiv 260240; doi: https://doi.org/10.1101/260240. <a href="https://doi.org/10.1101/260240" target="_blank">Link to preprint on bioRxiv service.</a></p>
dc.identifier.doi10.1101/260240
dc.identifier.urihttp://hdl.handle.net/20.500.14038/29317
dc.description.abstractProper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.
dc.language.isoen_US
dc.rightsThe copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY 4.0 International license.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectgenetics
dc.subjectTRIM-NHL
dc.subjectproteins
dc.subjectNHL-2
dc.subjectCSR-1
dc.subjectHRDE-1
dc.subject22G-RNA
dc.subjectgermline
dc.subjectgene expression
dc.subjectAmino Acids, Peptides, and Proteins
dc.subjectBiochemistry
dc.subjectGenetic Phenomena
dc.subjectGenetics
dc.subjectMolecular Biology
dc.subjectNucleic Acids, Nucleotides, and Nucleosides
dc.titleThe TRIM-NHL protein NHL-2 is a Novel Co-Factor of the CSR-1 and HRDE-1 22G-RNA Pathways [preprint]
dc.typePreprint
dc.source.journaltitlebioRxiv
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2549&amp;context=faculty_pubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/faculty_pubs/1543
dc.identifier.contextkey12314305
refterms.dateFOA2022-08-23T15:53:16Z
html.description.abstract<p>Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the <em>C. elegans</em> germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the <em>C. elegans</em> germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.</p>
dc.identifier.submissionpathfaculty_pubs/1543
dc.contributor.departmentProgram in Bioinformatics and Integrative Biology


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The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY 4.0 International license.
Except where otherwise noted, this item's license is described as The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY 4.0 International license.