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dc.contributor.authorAbramo, Kristin
dc.contributor.authorValton, Anne-Laure
dc.contributor.authorVenev, Sergey V.
dc.contributor.authorOzadam, Hakan
dc.contributor.authorFox, A. Nicole
dc.contributor.authorDekker, Job
dc.date2022-08-11T08:08:23.000
dc.date.accessioned2022-08-23T15:53:35Z
dc.date.available2022-08-23T15:53:35Z
dc.date.issued2019-06-21
dc.date.submitted2019-06-26
dc.identifier.citation<p>bioRxiv 678474; doi: https://doi.org/10.1101/678474. <a href="https://doi.org/10.1101/678474" target="_blank">Link to preprint on bioRxiv service.</a></p>
dc.identifier.doi10.1101/678474
dc.identifier.urihttp://hdl.handle.net/20.500.14038/29384
dc.description.abstractChromosome folding is extensively modulated as cells progress through the cell cycle. During mitosis, condensin complexes fold chromosomes in helically arranged nested loop arrays. In interphase, the cohesin complex generates loops that can be stalled at CTCF sites leading to positioned loops and topologically associating domains (TADs), while a separate process of compartmentalization drives the spatial segregation of active and inactive chromatin domains. We used synchronized cell cultures to determine how the mitotic chromosome conformation is transformed into the interphase state. Using Hi-C, chromatin binding assays, and immunofluorescence we show that by telophase condensin-mediated loops are lost and a transient folding intermediate devoid of most loops forms. By late telophase, cohesin-mediated CTCF-CTCF loops and positions of TADs start to emerge rapidly. Compartment boundaries are also established in telophase, but long-range compartmentalization is a slow process and proceeds for several hours after cells enter G1. Our results reveal the kinetics and order of events by which the interphase chromosome state is formed and identify telophase as a critical transition between condensin and cohesin driven chromosome folding.
dc.language.isoen_US
dc.relationNow published in Nature Cell Biology doi: 10.1038/s41556-019-0406-2
dc.rightsThe copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectgenomics
dc.subjectchromosome folding
dc.subjectcondensin-to-cohesin transition
dc.subjecttelophase
dc.subjectinterphase
dc.subjectAmino Acids, Peptides, and Proteins
dc.subjectBioinformatics
dc.subjectComputational Biology
dc.subjectGenetic Phenomena
dc.subjectGenomics
dc.subjectMolecular Biology
dc.subjectStructural Biology
dc.titleA chromosome folding intermediate at the condensin-to-cohesin transition during telophase [preprint]
dc.typePreprint
dc.source.journaltitlebioRxiv
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2628&amp;context=faculty_pubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/faculty_pubs/1614
dc.identifier.contextkey14812741
refterms.dateFOA2022-08-23T15:53:35Z
html.description.abstract<p>Chromosome folding is extensively modulated as cells progress through the cell cycle. During mitosis, condensin complexes fold chromosomes in helically arranged nested loop arrays. In interphase, the cohesin complex generates loops that can be stalled at CTCF sites leading to positioned loops and topologically associating domains (TADs), while a separate process of compartmentalization drives the spatial segregation of active and inactive chromatin domains. We used synchronized cell cultures to determine how the mitotic chromosome conformation is transformed into the interphase state. Using Hi-C, chromatin binding assays, and immunofluorescence we show that by telophase condensin-mediated loops are lost and a transient folding intermediate devoid of most loops forms. By late telophase, cohesin-mediated CTCF-CTCF loops and positions of TADs start to emerge rapidly. Compartment boundaries are also established in telophase, but long-range compartmentalization is a slow process and proceeds for several hours after cells enter G1. Our results reveal the kinetics and order of events by which the interphase chromosome state is formed and identify telophase as a critical transition between condensin and cohesin driven chromosome folding.</p>
dc.identifier.submissionpathfaculty_pubs/1614
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.contributor.departmentProgram in Systems Biology


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The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.
Except where otherwise noted, this item's license is described as The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.