A DNA-guided Argonaute Protein Functions in DNA Replication in Thermus thermophilus [preprint]

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Authors
Jolly, Samson M.
Zhang, Han
Strittmatter, Lara
Hendricks, Gregory M.
Dhabaria, Avantika
Ueberheide, Beatrix
Zamore, Phillip D.
UMass Chan Affiliations
Graduate School of Biomedical Sciences
Department of Radiology
RNA Therapeutics Institute
Department of Biochemistry and Molecular Pharmacology
Document Type
Preprint
Publication Date
2019-12-09
Keywords
Microbiology
Argonaute Protein
DNA Replication
Thermus thermophilus
Amino Acids, Peptides, and Proteins
Biochemistry, Biophysics, and Structural Biology
Microbiology
Nucleic Acids, Nucleotides, and Nucleosides

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Abstract
Argonaute proteins use nucleic acid guides to protect organisms against transposons and viruses. In the eubacterium Thermus thermophilus, the DNA-guided Argonaute TtAgo defends against transformation by DNA plasmids. Here, we report that TtAgo also participates in DNA replication. TtAgo binds small DNA guides derived from the chromosomal region where replication terminates and associates with proteins known to act in DNA replication. T. thermophilus deploys a single type II topoisomerase, gyrase. When gyrase is inhibited, T. thermophilus relies on TtAgo to complete replication of its circular genome; loss of both gyrase and TtAgo activity produces long filaments that fail to separate into individual bacteria. We propose that the primary role of TtAgo is to help T. thermophilus disentangle the catenated circular chromosomes made by DNA replication.
Source

bioRxiv 869172; doi: https://doi.org/10.1101/869172. Link to preprint on bioRxiv service.
DOI
10.1101/869172
Permanent Link to this Item
http://hdl.handle.net/20.500.14038/29431
Related Resources
Now published in Cell doi: 10.1016/j.cell.2020.07.036
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The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.
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http://creativecommons.org/licenses/by-nc-nd/4.0/
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The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.
Except where otherwise noted, this item's license is described as The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.