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    Initial Kinetic Characterization of Sterile Alpha and Toll/Interleukin Receptor Motif-Containing Protein 1

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    Authors
    Loring, Heather S.
    Icso, Janneke D.
    Nemmara, Venkatesh V.
    Thompson, Paul R
    UMass Chan Affiliations
    Thompson Lab
    Graduate School of Biomedical Sciences
    Program in Chemical Biology
    Department of Biochemistry and Molecular Pharmacology
    Document Type
    Journal Article
    Publication Date
    2020-02-12
    Keywords
    Peptides and proteins
    Assays
    Inhibitors
    Inhibition
    Nicotinamide
    Amino Acids, Peptides, and Proteins
    Biochemistry
    Enzymes and Coenzymes
    Medicinal-Pharmaceutical Chemistry
    Molecular and Cellular Neuroscience
    Nervous System Diseases
    Nucleic Acids, Nucleotides, and Nucleosides
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    Link to Full Text
    https://doi.org/10.1021/acs.biochem.9b01078
    Abstract
    Sterile alpha and toll/interleukin receptor (TIR) motif-containing protein 1 (SARM1) plays a pivotal role in triggering the neurodegenerative processes that underlie peripheral neuropathies, traumatic brain injury, and neurodegenerative diseases. Importantly, SARM1 knockdown or knockout prevents degeneration, thereby demonstrating that SARM1 is a promising therapeutic target. Recently, SARM1 was shown to promote neurodegeneration via its ability to hydrolyze NAD(+), forming nicotinamide and ADP ribose (ADPR). Herein, we describe the initial kinetic characterization of full-length SARM1, as well as the truncated constructs corresponding to the SAM(1-2)TIR and TIR domains, highlighting the distinct challenges that have complicated efforts to characterize this enzyme. Moreover, we show that bacterially expressed full-length SARM1 (kcat/KM = 6000 +/- 2000 M(-1) s(-1)) is at least as active as the TIR domain alone (kcat/KM = 1500 +/- 300 M(-1) s(-1)). Finally, we show that the SARM1 hydrolyzes NAD(+) via an ordered uni-bi reaction in which nicotinamide is released prior to ADPR.
    Source

    Loring HS, Icso JD, Nemmara VV, Thompson PR. Initial Kinetic Characterization of Sterile Alpha and Toll/Interleukin Receptor Motif-Containing Protein 1. Biochemistry. 2020 Mar 3;59(8):933-942. doi: 10.1021/acs.biochem.9b01078. Epub 2020 Feb 17. PMID: 32049506. Link to article on publisher's site

    DOI
    10.1021/acs.biochem.9b01078
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/29438
    PubMed ID
    32049506
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    Link to Article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.1021/acs.biochem.9b01078
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    Morningside Graduate School of Biomedical Sciences Scholarly Publications
    UMass Chan Faculty and Researcher Publications
    Thompson Lab Publications

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