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dc.contributor.authorYu, Lijian
dc.contributor.authorVolkert, Michael R.
dc.date2022-08-11T08:08:24.000
dc.date.accessioned2022-08-23T15:54:07Z
dc.date.available2022-08-23T15:54:07Z
dc.date.issued2013-03-01
dc.date.submitted2013-07-09
dc.identifier.citation<p>Nucleic Acids Res. 2013 Mar 1;41(5):3104-14. doi: 10.1093/nar/gkt020. <a href="http://dx.doi.org/10.1093/nar/gkt020">Link to article on publisher's site</a></p>
dc.identifier.issn0305-1048 (Linking)
dc.identifier.doi10.1093/nar/gkt020
dc.identifier.pmid23355614
dc.identifier.urihttp://hdl.handle.net/20.500.14038/29486
dc.description.abstractAlternative polyadenylation (APA) is conserved in all eukaryotic cells. Selective use of polyadenylation sites appears to be a highly regulated process and contributes to human pathogenesis. In this article we report that the yeast RPB2 gene is alternatively polyadenylated, producing two mRNAs with different lengths of 3'UTR. In normally growing wild-type cells, polyadenylation preferentially uses the promoter-proximal poly(A) site. After UV damage transcription of RPB2 is initially inhibited. As transcription recovers, the promoter-distal poly(A) site is preferentially used instead, producing more of a longer form of RPB2 mRNA. We show that the relative increase in the long RPB2 mRNA is not caused by increased mRNA stability, supporting the preferential usage of the distal poly(A) site during transcription recovery. We demonstrate that the 3'UTR of RPB2 is sufficient for this UV-induced regulation of APA. We present evidence that while transcription initiation rates do not seem to influence selection of the poly(A) sites of RPB2, the rate of transcription elongation is an important determinant.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=23355614&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright The Author(s) 2013. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.subject3' Untranslated Regions
dc.subjectBase Sequence
dc.subject*DNA Damage
dc.subjectPolyadenylation
dc.subjectRNA Polymerase II
dc.subjectRNA, Fungal
dc.subjectRNA, Messenger
dc.subjectSaccharomyces cerevisiae
dc.subjectSaccharomyces cerevisiae Proteins
dc.subjectTranscription Elongation, Genetic
dc.subjectTranscription Initiation, Genetic
dc.subjectUltraviolet Rays
dc.subjectCellular and Molecular Physiology
dc.subjectFungi
dc.subjectGenetic Phenomena
dc.subjectMolecular Biology
dc.subjectMolecular Genetics
dc.subjectNucleic Acids, Nucleotides, and Nucleosides
dc.titleUV damage regulates alternative polyadenylation of the RPB2 gene in yeast
dc.typeJournal Article
dc.source.journaltitleNucleic acids research
dc.source.volume41
dc.source.issue5
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1170&amp;context=faculty_pubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/faculty_pubs/171
dc.identifier.contextkey4297381
refterms.dateFOA2022-08-23T15:54:07Z
html.description.abstract<p>Alternative polyadenylation (APA) is conserved in all eukaryotic cells. Selective use of polyadenylation sites appears to be a highly regulated process and contributes to human pathogenesis. In this article we report that the yeast RPB2 gene is alternatively polyadenylated, producing two mRNAs with different lengths of 3'UTR. In normally growing wild-type cells, polyadenylation preferentially uses the promoter-proximal poly(A) site. After UV damage transcription of RPB2 is initially inhibited. As transcription recovers, the promoter-distal poly(A) site is preferentially used instead, producing more of a longer form of RPB2 mRNA. We show that the relative increase in the long RPB2 mRNA is not caused by increased mRNA stability, supporting the preferential usage of the distal poly(A) site during transcription recovery. We demonstrate that the 3'UTR of RPB2 is sufficient for this UV-induced regulation of APA. We present evidence that while transcription initiation rates do not seem to influence selection of the poly(A) sites of RPB2, the rate of transcription elongation is an important determinant.</p>
dc.identifier.submissionpathfaculty_pubs/171
dc.contributor.departmentDepartment of Microbiology and Physiological Systems
dc.source.pages3104-14


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