IgA MAb blocks SARS-CoV-2 Spike-ACE2 interaction providing mucosal immunity [preprint]
Authors
Monir, EjemelLi, Qi
Hou, Shurong
Schiller, Zachary
Wallace, Aaron
Amcheslavsky, Alla
Yilmaz, Nese Kurt
Toomey, Jacqueline R.
Schneider, Ryan
Cavacini, Lisa
Klempner, Mark S.
Schiffer, Celia A.
Wang, Yan
UMass Chan Affiliations
Schiffer LabDepartment of Biochemistry and Molecular Pharmacology
MassBiologics
Document Type
PreprintPublication Date
2020-05-15Keywords
microbiologybiochemistry
SARS-CoV-2
mucosal immunity
antibody
COVID-19
Amino Acids, Peptides, and Proteins
Biochemistry
Immunity
Immunology of Infectious Disease
Immunoprophylaxis and Therapy
Immunotherapy
Infectious Disease
Microbiology
Virus Diseases
Metadata
Show full item recordAbstract
COVID-19 caused by SARS-CoV-2 has become a global pandemic requiring the development of interventions for the prevention or treatment to curtail mortality and morbidity. No vaccine to boost mucosal immunity or as a therapeutic has yet been developed to SARS-CoV-2. In this study we discover and characterize a cross-reactive human IgA monoclonal antibody, MAb362. MAb362 binds to both SARS-CoV and SARS-CoV-2 spike proteins and competitively blocks hACE2 receptor binding, by completely overlapping the hACE2 structural binding epitope. Furthermore, MAb362 IgA neutralizes both pseudotyped SARS-CoV and SARS-CoV-2 in human epithelial cells expressing hACE2. SARS-CoV-2 specific IgA antibodies, such as MAb362, may provide effective immunity against SARS-CoV-2 by inducing mucosal immunity within the respiratory system, a potentially critical feature of an effective vaccine.Source
Ejemel M, Li Q, Hou S, Schiller ZA, Wallace AL, Amcheslavsky A, Yilmaz NK, Toomey JR, Schneider R, Close BJ, Chen DY, Conway HL, Mohsan S, Cavacini LA, Klempner MS, Schiffer CA, Wang Y. IgA MAb blocks SARS-CoV-2 Spike-ACE2 interaction providing mucosal immunity. bioRxiv [Preprint]. 2020 May 15:2020.05.15.096719. doi: 10.1101/2020.05.15.096719. PMID: 32511396; PMCID: PMC7263543. Link to article on publisher's site
DOI
10.1101/2020.05.15.096719Permanent Link to this Item
http://hdl.handle.net/20.500.14038/29495PubMed ID
32511396Notes
Full author list omitted for brevity. For the full list of authors, see article.
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Rights
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.ae974a485f413a2113503eed53cd6c53
10.1101/2020.05.15.096719